Phospho-Syk (Y525 + Y526) Recombinant Rabbit Monoclonal Antibody [PSH03-70]
Catalog# HA722034
Phospho-Syk (Y525 + Y526) Recombinant Rabbit Monoclonal Antibody [PSH03-70]
-
WB
-
IF-Cell
-
Human
概述
产品名称
Phospho-Syk (Y525 + Y526) Recombinant Rabbit Monoclonal Antibody [PSH03-70]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Tyr525 and 526 of Human Syk.
种属反应性
Human
验证应用
WB, IF-Cell
分子量
Predicted band size: 72 kDa
阳性对照
Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate, Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes.
偶联
unconjugated
克隆号
PSH03-70
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IF-Cell
-
1:1,000
靶点
功能
Tyrosine-protein kinase SYK, also known as spleen tyrosine kinase, is an enzyme which in humans is encoded by the SYK gene. SYK, along with ZAP70, is a member of the Syk family of tyrosine kinases. These cytoplasmic non-receptor tyrosine kinases share a characteristic dual SH2 domain separated by a linker domain. However, activation of SYK relies less on phosphorylation by Src family kinases than ZAP70. SYK and ZAP70 share a common evolutionary origin and split from a common ancestor in the jawed vertebrates. While Syk and ZAP70 are primarily expressed in hematopoietic tissues, a variety of tissues express Syk. Within B and T cells, respectively, Syk and ZAP70 transmit signals from the B-cell receptor and T-cell receptor. Syk plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc receptor, and integrins.
背景文献
1. Wang S et al. TREM2 drives microglia response to amyloid-β via SYK-dependent and -independent pathways. Cell. 2022 Oct
2. Ennerfelt H et al. SYK coordinates neuroprotective microglial responses in neurodegenerative disease. Cell. 2022 Oct
亚细胞定位
Cell membrane, Cytoplasm, cytosol.
UNIPROT
别名
EC 2.7.10.2 antibody
kinase Syk antibody
KSYK antibody
KSYK_HUMAN antibody
p72-Syk antibody
p72syk antibody
Spleen tyrosine kinase antibody
Syk antibody
Tyrosine protein kinase SYK antibody
Tyrosine-protein kinase SYK antibody
图片
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Western blot analysis of Phospho-Syk (Y525 + Y526) on different lysates with Rabbit anti-Phospho-Syk (Y525 + Y526) antibody (HA722034) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: Lane 1-2 (left): 1 minute 2 seconds; Lane 1-2 (right): 2 minutes 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722034) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes labeling Phospho-Syk (Y525 + Y526) with Rabbit anti-Phospho-Syk (Y525 + Y526) antibody (HA722034) at 1/1,000 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Syk (Y525 + Y526) antibody (HA722034) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Syk Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P
Reactivity: Human,Rat
Conjugate: unconjugated
