Phospho-NF-kappaB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00]
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概述
产品名称
Phospho-NF-kappaB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser529 of Human NF-kB p65.
种属反应性
Human
验证应用
WB, IHC-P, FC, IP
分子量
Predicted band size: 60 kDa
阳性对照
HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes cell lysate, human lung tissue, human lung squamous cell carcinoma tissue, Daudi.
偶联
unconjugated
克隆号
SP07-00
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
-
FC
-
1:50
-
IP
-
Use at an assay dependent concentration.
发表文章中的应用
发表文章中的种属
Human | See 7 publications below |
Mouse | See 3 publications below |
Rat | See 2 publications below |
靶点
功能
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
背景文献
1. Yang K et al. Effect of PLCe gene silencing on inhibiting the cancerous transformation of ulcerative colitis. Exp Ther Med 12:422-426 (2016).
2. Jeon M et al. Elevated IL-1 expression induces invasiveness of triple negative breast cancer cells and is suppressed by zerumbone. Chem Biol Interact 258:126-33 (2016).
翻译后修饰
Ubiquitinated by RNF182, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.; Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes (By similarity).; Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity. Phosphorylation at Ser-276 by RPS6KA4 and RPS6KA5 promotes its transactivation and transcriptional activities.; Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3 and SIRT2. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation at Lys-310 promotes interaction with BRD4. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of Lys-310. Lys-310 is deacetylated by SIRT2.; S-nitrosylation of Cys-38 inactivates the enzyme activity.; Sulfhydration at Cys-38 mediates the anti-apoptotic activity by promoting the interaction with RPS3 and activating the transcription factor activity.; Sumoylation by PIAS3 negatively regulates DNA-bound activated NF-kappa-B.; Proteolytically cleaved within a conserved N-terminus region required for base-specific contact with DNA in a CPEN1-mediated manner, and hence inhibits NF-kappa-B transcriptional activity.
亚细胞定位
Nucleus, Cytoplasm.
UNIPROT #
别名
Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
MGC131774 antibody
NF kappa B p65delta3 antibody
NFKB3 antibody
Nuclear Factor NF Kappa B p65 Subunit antibody
Nuclear factor NF-kappa-B p65 subunit antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
OTTHUMP00000233473 antibody
OTTHUMP00000233474 antibody
展开Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
MGC131774 antibody
NF kappa B p65delta3 antibody
NFKB3 antibody
Nuclear Factor NF Kappa B p65 Subunit antibody
Nuclear factor NF-kappa-B p65 subunit antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
OTTHUMP00000233473 antibody
OTTHUMP00000233474 antibody
OTTHUMP00000233475 antibody
OTTHUMP00000233476 antibody
OTTHUMP00000233900 antibody
p65 antibody
p65 NF kappaB antibody
p65 NFkB antibody
relA antibody
TF65_HUMAN antibody
Transcription factor p65 antibody
v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
V rel avian reticuloendotheliosis viral oncogene homolog A antibody
v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody
Phospho-NF-κB p65 (S529)
Phospho NF κB p65 (S529)
Phospho-NF-κB p65 (S529)
折叠图片
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Western blot analysis of Phospho-NF-kappaB p65 (S529) on different lysates with Rabbit anti-Phospho-NF-kappaB p65 (S529) antibody (ET1604-27) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes cell lysate
Lane 3: HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 65 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Phospho-NF-kappaB p65 (S529) antibody (ET1604-27) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-27) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-Phospho-NF-kappaB p65 (S529) antibody (ET1604-27) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-27) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Phospho-NF-kappaB p65 (S529) was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1604-27, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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iPSC-derived NK cells with site-specific integration of CAR19 and IL24 at the multi-copy rDNA locus enhanced antitumor activity and proliferation
Author: Shi Qingxin,et al
PMID: 38737469
应用: WB
反应种属: Human
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Citation
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IGFBP7 promotes gastric cancer by facilitating epithelial-mesenchymal transition of gastric cells
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应用: WB
反应种属: Human
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Citation
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Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12
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PMID: NO PMID20240540
应用: WB
反应种属: Mouse
发表时间: 2024 May
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Citation
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Author: Zhang Baocheng,et al
PMID: 38411331
应用: WB
反应种属: Human
发表时间: 2024 Mar
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Citation
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应用: WB
反应种属: Human
发表时间: 2024 Aug
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Author: Yu A, Hu J, Fu L, Huang G, Deng D, Zhang M, Wang Y, Shu G, Jing L, Li H, Chen X, Yang T, Wei J, Chen Z, Zu X, Luo J
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应用: WB
反应种属: Mouse,Human
发表时间: 2023 Oct
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Citation
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Author: Li, J., Zhang, X., Guo, D., Shi, Y., Zhang, S., Yang, R., & Cheng, J.
PMID: 36992829
应用: IHC
反应种属: Rat
发表时间: 2023 Mar
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Citation
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NCOA2-induced secretion of leptin leads to fetal growth restriction via the NF-κB signaling pathway
Author:
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应用: WB
反应种属: human
发表时间: 2023 Feb
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反应种属: Mouse
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反应种属: Human
发表时间: 2022 Jun
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反应种属: Rabbit
发表时间: 2022 Aug
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miR‐20a‐5p regulated SMAD6 to inhibit chondrogenesis of hDPSCs
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应用:
反应种属:
发表时间: 2021 Sep
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Citation
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6-Gingerol attenuates ventilator-induced lung injury via anti-inflammation and antioxidative stress by modulating the pparγ/nf-κb signalling pathway in rats
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应用: WB
反应种属: Rat
发表时间: 2021 Mar
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Citation
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MGP Promotes Colon Cancer Proliferation by Activating the NF-kB Pathway through Upregulation of the Calcium Signaling Pathway
Author:
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应用: WB,IF
反应种属:
发表时间: 2020 Apr
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Citation
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Citation
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