TrkB Recombinant Rabbit Monoclonal Antibody [JE58-22]
Catalog# ET7111-38
TrkB Recombinant Rabbit Monoclonal Antibody [JE58-22]
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WB
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IHC-P
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Mouse
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Rat
概述
产品名称
TrkB Recombinant Rabbit Monoclonal Antibody [JE58-22]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human TrkB aa 100-450/822.
种属反应性
Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 92 kDa
阳性对照
Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, rat brain tissue.
偶联
unconjugated
克隆号
JE58-22
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
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IHC-P
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1:200
发表文章中的应用
| WB | 查看 2 篇文献如下 |
发表文章中的种属
| Rat | 查看 1 篇文献如下 |
| Mouse | 查看 1 篇文献如下 |
靶点
功能
Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
背景文献
1. Wang X. et. al. A tropomyosin receptor kinase family protein, NTRK2 is a potential predictive biomarker for lung adenocarcinoma. PeerJ. 2019 Jun
2. Cocco E. et. al. NTRK fusion-positive cancers and TRK inhibitor therapy. Nat Rev Clin Oncol. 2018 Dec
亚细胞定位
Cell membrane, Endosome membrane, Early endosome membrane, axon, dendrite, perinuclear region, postsynaptic density.
别名
AI848316 antibody
BDNF tropomyosine receptor kinase B antibody
BDNF/NT 3 growth factors receptor antibody
BDNF/NT-3 growth factors receptor antibody
Brain derived neurotrophic factor receptor antibody
C030027L06Rik antibody
EC 2.7.10.1 antibody
GP145 TrkB antibody
GP145-TrkB antibody
GP145-TrkB/GP95-TrkB antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of TrkB on different lysates with Rabbit anti-TrkB antibody (ET7111-38) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse liver tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-38) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TrkB antibody (ET7111-38) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-38) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Potential value of Interleukin-6 as a diagnostic biomarker in human MDD and the antidepressant effect of its receptor antagonist tocilizumab in lipopolysaccharide-challenged rats
Author: Xu D, Xu Y, Gao X, et al
PMID: 37717319
应用: WB
反应种属: Rat
发表时间: 2023 Nov
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Citation
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Icariin improves learning and memory function in Aβ1-42-induced AD mice through regulation of the BDNF-TrκB signaling pathway
Author: Meiyu Wan, Shengqi Sun, Xiaoke Di, Minghui Zhao, Fengjuan Lu, Zhifei Zhang, Yang Li
PMID: 37579923
应用: WB
反应种属: Mouse
发表时间: 2023 Aug
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Citation
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