TRF1 Recombinant Rabbit Monoclonal Antibody [JE59-70]
Catalog# HA721505
TRF1 Recombinant Rabbit Monoclonal Antibody [JE59-70]
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WB
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Human
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Mouse
概述
产品名称
TRF1 Recombinant Rabbit Monoclonal Antibody [JE59-70]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human TRF1 aa 51-150 / 439.
种属反应性
Human, Mouse
验证应用
WB
分子量
Predicted band size: 50 kDa
阳性对照
Jurkat cell lysate, K-562 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, RAW264.7 cell lysate.
偶联
unconjugated
克隆号
JE59-70
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
The human TERF1 gene is located in the chromosome 8 at 73,921,097-73,960,357 bp. Two transcripts of this gene are alternatively spliced products.[6] The TERF1 gene is also known as TRF, PIN2 (Proteinase Inhibitor 2), TRF1, t-TRF1 and h-TRF1-AS. TERF 1 gene encodes a telomere specific protein which is a component of the telomere's shelterin nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. It is known to protect telomeres in mammals from DNA mechanisms that are used for repair purposes and at the same time regulate the activity carried out by telomerase. The telomeric repeat binding factor 1 protein is present at telomeres, where the cells aging aspect is monitored, throughout the typical cell cycle process. The progressive loss of the telomeric ends of chromosomes is an important mechanism in the timing of human cellular aging. Telomeric Repeat Factor 1 (TRF1) is a protein that binds at telomere ends. The protein has the ultimate use of functioning as an inhibitor of telomerase, a protein enzyme that assists in the elongation of chromosomes by the addition of sequences of TTAGGG to the end of the chromosomes. The protein acts as cis-regulatory elements in the process of limiting the ends of individual chromosomes from elongating as facilitated by telomerase and the TTAGGG sequences. The structure of the protein consists of a dimerization domain close to its amino terminus, a carboxyl terminal tail, which is the free carboxyl group that terminates the end of a protein chain and an acidic amino terminus, which is the free amine group that terminates the start of a protein.
背景文献
1. Yang Z et al. TRF1 uses a noncanonical function of TFIIH to promote telomere replication. Genes Dev. 2022 Sep
2. Li X et al. Dynamics of TRF1 organizing a single human telomere. Nucleic Acids Res. 2021 Jan
亚细胞定位
Nucleus, Cytoplasm, cytoskeleton, spindle, Chromosome, telomere.
别名
hTRF1 AS antibody
NIMA interacting protein 2 antibody
NIMA-interacting protein 2 antibody
PIN 2 antibody
PIN2 antibody
t TRF1 antibody
Telomeric protein Pin2 antibody
Telomeric protein Pin2/TRF1 antibody
Telomeric repeat binding factor (NIMA interacting) 1 antibody
Telomeric repeat binding factor 1 antibody
展开图片
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Western blot analysis of TRF1 on different lysates with Rabbit anti-TRF1 antibody (HA721505) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: MCF7 cell lysate
Lane 5: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721505) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"