Skip to content

TIM 3 Mouse Monoclonal Antibody [1E5]

RMB: 900 特惠 1500

产品规格

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# EM1701-20

TIM 3 Mouse Monoclonal Antibody [1E5]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

TIM 3 Mouse Monoclonal Antibody [1E5]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein with Human TIM 3 1-89 / 301.

种属反应性

Human

验证应用

WB, IF-Cell, IHC-P, FC

分子量

Predicted band size: 33 kDa

阳性对照

THP-1 cell lysate, A549 cell lysate, HepG2 cell lysate, human lung cancer tissue, human spleen tissue, human tonsil tissue, 293T, Daudi.

偶联

unconjugated

克隆号

1E5

RRID

AB_3068748

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:50-1:100

发表文章中的种属

Human 查看 2 篇文献如下

靶点

功能

Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse.

背景文献

1. Weber JK et al. Phosphatidylserine-Induced Conformational Modulation of Immune Cell Exhaustion-Associated Receptor TIM3. Sci Rep 7(1):13579 (2017).

2. Tao J et al. Increased TIM3+CD8+T cells in Myelodysplastic Syndrome patients displayed less perforin and granzyme B secretion and higher CD95 expression. Leuk Res 51:49-55 (2016).

序列相似性

Belongs to the immunoglobulin superfamily. TIM family.

组织特异性

Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.

翻译后修饰

O-glycosylated with core 1 or possibly core 8 glycans.; Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9.

亚细胞定位

Membrane. Cell junction.

UNIPROT

SwissProt: Q8TDQ0 Human

别名

CD366 antibody

FLJ14428 antibody

HAVcr-2 antibody

Havcr2 antibody

HAVR2_HUMAN antibody

Hepatitis A virus cellular receptor 2 antibody

Kidney injury molecule 3 antibody

KIM 3 antibody

KIM3 antibody

T cell immunoglobulin and mucin domain containing 3 antibody

展开

图片

  • Western blot analysis of TIM 3 on different lysates with Mouse anti-TIM 3 antibody (<a href="/products/EM1701-20" style="font-weight: bold;text-decoration: underline;">EM1701-20</a>) at 1/2,000 dilution.<br /><br />Lane 1: THP-1 cell lysate (no heat)<br />Lane 2: A549 cell lysate (no heat)<br />Lane 3: HepG2 cell lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 5 minutes 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-20" style="font-weight: bold;text-decoration: underline;">EM1701-20</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TIM 3 on different lysates with Mouse anti-TIM 3 antibody (EM1701-20) at 1/2,000 dilution.

    Lane 1: THP-1 cell lysate (no heat)
    Lane 2: A549 cell lysate (no heat)
    Lane 3: HepG2 cell lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 42 kDa

    Exposure time: 5 minutes 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-20) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

  • Immunocytochemistry analysis of 293T cells labeling Tim3 with Mouse anti-Tim3 antibody (<a href="/products/EM1701-20" style="font-weight: bold;text-decoration: underline;">EM1701-20</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Tim3 antibody (<a href="/products/EM1701-20" style="font-weight: bold;text-decoration: underline;">EM1701-20</a>) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of 293T cells labeling Tim3 with Mouse anti-Tim3 antibody (EM1701-20) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Tim3 antibody (EM1701-20) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of Daudi cells with Tim3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Daudi cells with Tim3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
  2. IHC-P
  3. IF
Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单