SV2B Rabbit Polyclonal Antibody
Catalog# ER1917-37
SV2B Rabbit Polyclonal Antibody
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WB
-
IHC-P
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IF-Tissue
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Human
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Mouse
-
Rat
概述
产品名称
SV2B Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human SV2B 401-500/683
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue
分子量
77 KDa
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-2000
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IHC-P
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1:100-500
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IF-tissue
-
1:100-500
靶点
功能
SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. While SV2A is expressed ubiquitously throughout the brain, SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern.
背景文献
暂无
亚细胞定位
Cytoplasmic vesicle, secretory vesicle. Synaptic vesicle membrane; Multi-pass membrane protein.
UNIPROT
别名
Synaptic vesicle protein 2B antibody
图片
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Sample:
U251 Cell (Human) Lysate at 30 ug
Primary: Anti- SV2B (ER1917-37) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 77 kD -
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- SV2B (ER1917-37) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 77 kD -
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SV2B) Polyclonal Antibody, Unconjugated (ER1917-37) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
SV2B Mouse Monoclonal Antibody [A4C1]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Rat
Conjugate: unconjugated
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