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PBK Mouse Monoclonal Antibody [E11-C8]

RMB: 1500

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Catalog# EM1710-21

PBK Mouse Monoclonal Antibody [E11-C8]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

概述

产品名称

PBK Mouse Monoclonal Antibody [E11-C8]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein

种属反应性

Human

验证应用

WB, IF-Cell, IHC-P, FC

分子量

36 kDa

阳性对照

Human PBK recombinant protein, A431, Hela, human ovarian cancer tissue, human colon cancer tissue.

偶联

unconjugated

克隆号

E11-C8

产品特性

形态

Liquid

浓度

2ug/ul

存放说明

4℃; -20℃ for long term storage.

存储缓冲液

Purified antibody in PBS with 0.05% sodium azide.

亚型

IgG2b

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:500

  • IF-cell

  • 1:100-1:500

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:100-1:200

靶点

功能

Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Kinases are capable of influencing the oncogenic potential of cell sytems at the level of oncoprotein or tumor suppressor protein phosphorylation states. Human PDZ-binding kinase, known as PBK, is a 322 amino acid, T/SXV motif-containing serine/threonine kinase that is abuntant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. The cell cycle checkpoint kinase Cdc2/cyclin B is an upstream effector of PBK that can phosphorylate and activate PBK. Active PBK may associate with PDZ-containing proteins and influence cell cycle control or cellular proliferation.

背景文献

暂无

亚细胞定位

Nucleus.

UNIPROT #

SwissProt: Q96KB5 Human

别名

Cancer/testis antigen 84 antibody

CT84 antibody

Epididymis luminal protein 164 antibody

FLJ14385 antibody

HEL164 antibody

Lymphokine activated killer T cell originated protein kinase antibody

Lymphokine-activated killer T-cell-originated protein kinase antibody

MAPKK like protein kinase antibody

MAPKK-like protein kinase antibody

Nori 3 antibody

展开

图片

  • Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1,000 dilution.

    Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1,000 dilution.

  • Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1,000 dilution.

    Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1,000 dilution.

  • ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.

  • Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

    Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"