Nicastrin Recombinant Rabbit Monoclonal Antibody [JG68-33]
Catalog# ET7108-38
Nicastrin Recombinant Rabbit Monoclonal Antibody [JG68-33]
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WB
-
Human
-
Mouse
-
Rat
预测的种属支持售后
概述
产品名称
Nicastrin Recombinant Rabbit Monoclonal Antibody [JG68-33]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recomninant protein within Human Nicastrin aa 5-112 / 709.
种属反应性
Human, Mouse (Predicted: Rat)
预测的种属支持售后
验证应用
WB
分子量
Predicted band size: 78 kDa
阳性对照
THP-1 cell lysates, NIH/3T3 cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate.
偶联
unconjugated
克隆号
JG68-33
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
靶点
功能
The Presenilin 1 (PS1) and Presenilin 2 (PS2) transmembrane proteins are components of high molecular weight complexes. These complexes mediate proteolytic cleavage within the transmembrane domain of several proteins, including the β-Amyloid precursor protein (βAPP) and Notch. Missense mutations in the genes encoding the Presenilin proteins increase the proteolysis of βAPP and results in the overproduction of the neurotoxic β-Amyloid peptide, which results in a condition associated with Familial Alzheimer's disease (FAD). A novel component of the presenilin complex, nicastrin, is a type I transmembrane glycoprotein that is involved in mediating Notch/GLP-1 signaling. In addition, nicastrin contributes to the processing of βAPP, which makes nicastrin an attractive potential target for modulating the production of β-Amyloid in patients with Alzheimer's disease. Originally purified from immunoprecipitated PS1 complexes from HEK293 cells, nicastrin contains hydrophilic amino and carboxy-terminal domains, a short, hydrophobic transmembrane domain and potential N-myristoylation and phosphorylation sites.
背景文献
1. Yu G et al. Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. Nature 407:48-54 (2000).
2. Lu P et al. Three-dimensional structure of human gamma-secretase. Nature 512:166-170 (2014).
序列相似性
Belongs to the nicastrin family.
组织特异性
Detected in brain (at protein level). Widely expressed.
翻译后修饰
N-glycosylated.
亚细胞定位
Membrane, Cytoplasmic vesicle membrane, Melanosome.
别名
Anterior pharynx defective 2 antibody
APH 2 antibody
APH2 antibody
ATAG1874 antibody
KIAA0253 antibody
Ncstn antibody
NCT antibody
NICA_HUMAN antibody
Nicastrin antibody
RP11 517F10.1 antibody
展开图片
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Western blot analysis of Nicastrin on THP-1 cell lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 78-150 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: Neuro-2a cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 78 kDa
Observed band size: 78-150 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Nicastrin KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 78-150 kDa
Exposure time: 60 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"