Lamin B2 Mouse Monoclonal Antibody [D5-C2]
Catalog# M1505-11
Lamin B2 Mouse Monoclonal Antibody [D5-C2]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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Zebrafish
概述
产品名称
Lamin B2 Mouse Monoclonal Antibody [D5-C2]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human Lamin B2 aa 571-620 / 620.
种属反应性
Human, Mouse, Rat, Zebrafish
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 70 kDa
阳性对照
Jurkat cell lysates, hybrid fish (crucian-carp) brain tissue lysates, human uterine tissue.
偶联
unconjugated
克隆号
D5-C2
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:200-1:500
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IHC-P
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1:500-1:1000
靶点
功能
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
背景文献
1. "Sequencing of the reannotated LMNB2 gene reveals novel mutations in patients with acquired partial lipodystrophy." Hegele R.A., Cao H., Liu D.M., Costain G.A., Charlton-Menys V., Rodger N.W., Durrington P.N. Am. J. Hum. Genet. 79:383-389(2006)
2. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011)
序列相似性
Belongs to the intermediate filament family.
翻译后修饰
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
亚细胞定位
Nucleus inner membrane
UNIPROT
别名
Lamin-B2 antibody
Lamin B2 antibody
LMN 2 antibody
LMN B2 antibody
LMN2 antibody
LMNB 2 antibody
LMNB2 antibody
LMNB2_HUMAN antibody
MGC2721 antibody
RGD1563803 antibody
图片
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Western blot analysis of Lamin B2 on Jurkat cell lysate using anti-Lamin B2 antibody at 1/500 dilution.
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Western blot analysis of Lamin B2 on zebrafish tissue lysate using anti-Lamin B2 antibody at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded human uterine tissue with Mouse anti-Lamin B2 antibody (M1505-11) at 1/1000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-11) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
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Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
