IL-13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89]
Catalog# ET7106-61
IL-13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89]
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WB
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IF-Cell
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IHC-P
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Human
概述
产品名称
IL-13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody [JU38-89]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human IL13 Receptor alpha 1 aa 1-127 / 427.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 49 kDa
阳性对照
HepG2 cell lysate, HeLa cell lysate, HepG2, human liver tissue.
偶联
unconjugated
克隆号
JU38-89
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:50-1:200
靶点
功能
Interleukin 13 receptor, alpha 1, also known as IL13RA1 and CD213A1 (cluster of differentiation 213A1), is a human gene. Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
背景文献
1. Aguirre CA. et. al. Two single nucleotide polymorphisms in IL13 and IL13RA1 from individuals with idiopathic Parkinson\'s disease increase cellular susceptibility to oxidative stress. Brain Behav Immun. 2020 Aug
序列相似性
Belongs to the type I cytokine receptor family. Type 5 subfamily.
组织特异性
Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.
亚细胞定位
Membrane.
UNIPROT
别名
bB128O4.2.1 (interleukin 13 receptor, alpha 1) antibody
Cancer/testis antigen 19 antibody
CD213a1 antibody
CD213A1 Antigen antibody
CT 19 antibody
CT19 antibody
I13R1_HUMAN antibody
IL 13 receptor subunit alpha 1 antibody
IL 13R alpha 1 antibody
IL 13R subunit alpha 1 antibody
展开图片
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Western blot analysis of IL-13 Receptor alpha 1 on different lysates with Rabbit anti-IL-13 Receptor alpha 1 antibody (ET7106-61) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 10 second; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-61) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of IL-13 Receptor alpha 1 on different lysates with Rabbit anti-IL-13 Receptor alpha 1 antibody (ET7106-61) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-IL-13 Receptor alpha 1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 60 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-61) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of IL-13 Receptor alpha 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7106-61, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL-13 Receptor alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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