Hex Recombinant Rabbit Monoclonal Antibody [JE37-45]
Catalog# HA722753
Hex Recombinant Rabbit Monoclonal Antibody [JE37-45]
-
WB
-
IP
-
Human
概述
产品名称
Hex Recombinant Rabbit Monoclonal Antibody [JE37-45]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human Hex aa 91-240 / 270.
种属反应性
Human
验证应用
WB, IP
分子量
Predicted band size: 30 kDa
阳性对照
HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, HT-29 cell lysate.
偶联
unconjugated
克隆号
JE37-45
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IP
-
1-2μg/sample
靶点
功能
Hematopoietically-expressed homeobox protein HHEX is a protein that in humans is encoded by the HHEX gene and also known as Proline Rich Homeodomain protein PRH. This gene encodes a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation but the expression of this protein is not limited to hematopoietic cells.
背景文献
1. Guo Y et al. CK2-induced cooperation of HHEX with the YAP-TEAD4 complex promotes colorectal tumorigenesis. Nat Commun. 2022 Aug
2. Xu Z et al. HHEX suppresses advanced thyroid cancer by interacting with TLE3. Mol Cell Endocrinol. 2023 Aug
亚细胞定位
Nucleus, nuclear body, Cytoplasm.
UNIPROT
别名
Hematopoietically expressed homeobox antibody
Hematopoietically-expressed homeobox protein HHEX antibody
HEX antibody
HHEX antibody
HHEX_HUMAN antibody
HMPH antibody
Homeobox hematopoietically expressed antibody
Homeobox protein HEX antibody
Homeobox protein PRH antibody
HOX11L PEN antibody
展开图片
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Western blot analysis of Hex on different lysates with Rabbit anti-Hex antibody (HA722753) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HT-29 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 32 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722753) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Hex was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA722753 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722753 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/100,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate (input)
Lane 2: HA722753 IP in MCF7 cell lysate
Lane 3: Rabbit IgG instead of HA722753 in MCF7 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 8 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"