HSP47 Rabbit Polyclonal Antibody
Catalog# R1511-11
HSP47 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
HSP47 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within Human HSP47 aa 72-318 / 418.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 46 kDa
阳性对照
HeLa cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, rat placenta tissue lysate, Siha cell lysate, human placenta tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:500
发表文章中的应用
| WB | 查看 1 篇文献如下 |
发表文章中的种属
| Human | 查看 1 篇文献如下 |
靶点
功能
Heat shock protein 47, also known as SERPINH1 is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. HSP47 is expressed by cells present in Endoplasmic Reticulum. These cells synthesize and secrete type I and type II collagen. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. Heat shock protein 47 has been shown to interact with collagens I, II, III, IV and V. In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X. Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas. Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.
背景文献
1. Williams, R. Sanders. Heat Shock Protein 47: A Chaperone for the Fibrous Cap?. Circulation. 101 (11): 1227–1228 (21 March 2000).
2. Dafforn TR, Della M, Miller AD. The molecular interactions of heat shock protein 47 (Hsp47) and their implications for collagen biosynthesis. The Journal of Biological Chemistry. 276 (52): 49310–9 (December 2001).
序列相似性
Belongs to the serpin family.
亚细胞定位
Endoplasmic reticulum.
别名
47 kDa heat shock protein antibody
47 kDa heat shock protein precursor antibody
Arsenic transactivated protein 3 antibody
Arsenic-transactivated protein 3 antibody
AsTP 3 antibody
AsTP3 antibody
CBP 1 antibody
CBP 2 antibody
CBP1 antibody
CBP2 antibody
展开图片
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Western blot analysis of HSP47 on different lysates with Rabbit anti-HSP47 antibody (R1511-11) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: C2C12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of HSP47 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1511-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat placenta tissue lysate
Lane 2: Siha cell lysate
Lane 3: NIH/3T3 cell lysate -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HSP47 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-11, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Immune microenvironment infiltration landscape and immune-related subtypes in prostate cancer
Author: Wu, W., Wang, X., Le, W., Lu, C., Li, H., Zhu, Y., Chen, X., An, W., Xu, C., Wu, Q., & Wang, L.
PMID: 36700224
期刊: Frontiers In Immunology
应用: WB
反应种属: Human
发表时间: 2023 Jan
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Citation