ERp72 Recombinant Rabbit Monoclonal Antibody [JE77-55]
Catalog# HA722749
ERp72 Recombinant Rabbit Monoclonal Antibody [JE77-55]
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WB
-
IHC-P
-
Human
概述
产品名称
ERp72 Recombinant Rabbit Monoclonal Antibody [JE77-55]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 73 kDa
阳性对照
HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, human liver cancer tissue, human lung cancer tissue.
偶联
unconjugated
克隆号
JE77-55
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:200-1:1,000
靶点
功能
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly.
背景文献
1. Khalaf NB et al. Vascular endothelial ERp72 is involved in the inflammatory response in a rat model of skeletal muscle injury. Mol Med Rep. 2021 Mar
2. Matsusaki M et al. Functional Interplay between P5 and PDI/ERp72 to Drive Protein Folding. Biology (Basel). 2021 Oct
亚细胞定位
Endoplasmic reticulum lumen, Melanosome.
UNIPROT
别名
Calcium binding protein intestinal related antibody
Endoplasmic reticulum resident protein 70 antibody
Endoplasmic reticulum resident protein 72 antibody
EPR70 antibody
EPR72 antibody
ER protein 70 antibody
ER protein 72 antibody
ERP 70 antibody
ERp 72 antibody
ERp-72 antibody
展开图片
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Western blot analysis of ERp72 on different lysates with Rabbit anti-ERp72 antibody (HA722749) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722749) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-ERp72 antibody (HA722749) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722749) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-ERp72 antibody (HA722749) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722749) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"