Citrate synthetase Recombinant Rabbit Monoclonal Antibody [JU80-30]
Catalog# ET1706-40
Citrate synthetase Recombinant Rabbit Monoclonal Antibody [JU80-30]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
概述
产品名称
Citrate synthetase Recombinant Rabbit Monoclonal Antibody [JU80-30]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Citrate synthetase aa 417-466 / 466.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, FC
分子量
52 kDa
阳性对照
Rat kidney tissue lysate, Hela cell lysate, mouse brain tissue lysate, rat epididymis tissue, human tonsil tissue, human kidney tissue, 293T.
偶联
unconjugated
克隆号
JU80-30
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:50-1:100
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FC
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1:50-1:200
靶点
功能
Citrate synthase is found in nearly all cells capable of oxidative metabolism.The enzyme citrate synthase exists in nearly all living cells and stands as a pace-making enzyme in the first step of the citric acid cycle (or Krebs cycle). Citrate synthase is localized within eukaryotic cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria.
背景文献
1. Redmann M et al. Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons. Redox Biol 11:73-81 (2017).
2. Giroud M et al. Let-7i-5p represses brite adipocyte function in mice and humans. Sci Rep 6:28613 (2016).
序列相似性
Belongs to the citrate synthase family.
翻译后修饰
Methylated. Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity.
亚细胞定位
Mitochondrion matrix.
别名
CISY_HUMAN antibody
Citrate synthase antibody
Citrate synthase, mitochondrial antibody
citrate synthetase antibody
Cs antibody
EC 2.3.3 antibody
EC 2.3.3.1 antibody
图片
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☑ Knockdown (KD)
Western blot analysis of Citrate synthetase on different lysates with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Citrate synthetase KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 45 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-40) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Citrate synthetase on different lysates with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/1,000 dilution.
Lane 1: Rat kidney tissue lysates (20 µg/Lane)
Lane 2: Hela cell lysates
Lane 3: Mouse brain tissue lysates (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-40) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of 293T cells with Citrate synthetase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"