CD42a Recombinant Mouse Monoclonal Antibody [A5E6-R]
Catalog# HA601262
CD42a Recombinant Mouse Monoclonal Antibody [A5E6-R]
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WB
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IHC-P
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Human
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Mouse
-
Rat
概述
产品名称
CD42a Recombinant Mouse Monoclonal Antibody [A5E6-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within N-terminal human CD42a.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 19 kDa
阳性对照
MCF7 cell lysate, human lung tissue lysate, mouse spleen tissue lysate, mouse lung tissue lysate, mouse spleen tissue, rat spleen tissue.
偶联
unconjugated
克隆号
A5E6-R
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:2,000
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IHC-P
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1:2,000
靶点
功能
Glycoprotein IX (platelet) (GP9) also known as CD42a (Cluster of Differentiation 42a), is a human gene. Platelet glycoprotein IX (GP9) is a small membrane glycoprotein found on the surface of human platelets. It forms a 1-to-1 noncovalent complex with glycoprotein Ib (GP Ib), a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor(known as the Glycoprotein Ib-IX-V Receptor Complex). The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain and a beta chain, that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein.
背景文献
1. Martins Castanheira N et al. Uptake of platelets by cancer cells and recycling of the platelet protein CD42a. J Thromb Haemost. 2022 Jan
亚细胞定位
Membrane.
别名
CD42a antibody
Glycoprotein 9 antibody
Glycoprotein IX antibody
Glycoprotein IX (platelet) antibody
Glycoprotein IX precursor antibody
GP IX antibody
GP-IX antibody
GP9 antibody
GPIX antibody
GPIX_HUMAN antibody
展开图片
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Western blot analysis of CD42a on different lysates with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: Human lung tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Mouse lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 28 kDa
Exposure time: 1 minute 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601262) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601262) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-CD42a antibody (HA601262) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601262) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
CD42a Recombinant Mouse Monoclonal Antibody [A5E6-R] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
