SALL4 Mouse Monoclonal Antibody [A9G9]
Catalog# HA601144
SALL4 Mouse Monoclonal Antibody [A9G9]
-
IHC-P
-
WB
-
FC
-
IF-Tissue
-
Human
概述
产品名称
SALL4 Mouse Monoclonal Antibody [A9G9]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein within human SALL4 aa 904-1,053/1,053.
种属反应性
Human
验证应用
IHC-P, WB, FC, IF-Tissue
分子量
Predicted band size: 112 kDa
阳性对照
Human seminoma tissue, human testis tissue, NCCIT cell lysates, NCCIT.
偶联
unconjugated
克隆号
A9G9
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
-
1:4,000
-
WB
-
1:1,000
-
FC
-
1:500-1:1,000
-
IF-Tissue
-
1:500
靶点
功能
Sal-like protein 4 (SALL4) is a transcription factor encoded by a member of the Spalt-like (SALL) gene family, SALL4. The SALL genes were identified based on their sequence homology to Spalt, which is a homeotic gene originally cloned in Drosophila melanogaster that is important for terminal trunk structure formation in embryogenesis and imaginal disc development in the larval stages. There are four human SALL proteins (SALL1, 2, 3, and 4) with structural homology and playing diverse roles in embryonic development, kidney function, and cancer. The SALL4 gene encodes at least three isoforms, termed A, B, and C, through alternative splicing, with the A and B forms being the most studied. SALL4 can alter gene expression changes through its interaction with many co-factors and epigenetic complexes. It is also known as a key embryonic stem cell (ESC) factor.
背景文献
1. Sun B et al. SALL4 Oncogenic Function in Cancers: Mechanisms and Therapeutic Relevance. Int J Mol Sci. 2022 Feb
2. Moein S et al. SALL4: An Intriguing Therapeutic Target in Cancer Treatment. Cells. 2022 Aug
亚细胞定位
Cytoplasm, Nucleus.
UNIPROT
别名
AA407717 antibody
AL022809 antibody
AW536104 antibody
C330011P20Rik antibody
C78083 antibody
C78563 antibody
dJ1112F19.1 antibody
DRRS antibody
HSAL4 antibody
Sal like 4 (Drosophila) antibody
展开图片
-
Immunohistochemical analysis of paraffin-embedded human seminoma tissue with Mouse anti-SALL4 antibody (HA601144) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601144) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-SALL4 antibody (HA601144) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601144) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human appendix tissue (Negative) with Mouse anti-SALL4 antibody (HA601144) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601144) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of SALL4 on NCCIT cell lysates with Mouse anti-SALL4 antibody (HA601144) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 112 kDa
Observed band size: 150/110/70 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601144) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of NCCIT cells labeling SALL4.
Cells were fixed and permeabilized.Then stained with the primary antibody (HA601144, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Application: IF-Tissue
Species: Human
Site: seminoma
Sample: Paraffin-embedded section
Antibody concentration: 1/500
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"