RPA1 Mouse Monoclonal Antibody [D9-C4]
Catalog# EM1709-52
RPA1 Mouse Monoclonal Antibody [D9-C4]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Monkey
概述
产品名称
RPA1 Mouse Monoclonal Antibody [D9-C4]
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein
种属反应性
Human, Monkey
验证应用
WB, IF-Cell, IHC-P, FC
分子量
70 kDa
阳性对照
Human RPA1 recombinant protein, Hela, MCF-7, K562, A431, COS-7, colon cancer tissue, prostate tissue, Jurkat.
偶联
unconjugated
克隆号
D9-C4
产品特性
形态
Liquid
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-cell
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1:100-1:500
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IHC-P
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1:100-1:500
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FC
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1:100-1:200
靶点
功能
RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The RPA 70 kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins. The DNA binding domain lies in the middle of RPA 70 kDa subunit and comprises two structurally homologous subdomains oriented in tandem. RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.
背景文献
暂无
亚细胞定位
Nucleus.
UNIPROT
别名
Dmrpa1 antibody
Drosophila Replication Protein A antibody
DRPA antibody
HSSB antibody
Human single stranded DNA binding protein antibody
MST075 antibody
MSTP075 antibody
p70 antibody
REPA1 antibody
Replication factor A antibody
展开图片
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Western blot analysis of RPA70 on human RPA70 recombinant protein using anti-RPA70 antibody at 1/1,000 dilution.
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Western blot analysis of RPA70 on different cell lysates using anti-RPA70 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: K562
Lane 4: A431
Lane 5: COS-7 -
ICC staining RPA70 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-RPA70 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded prostate tissue using anti-RPA70 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of Jurkat cells with RPA70 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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