概述
产品名称
Osterix Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human Osterix 331-431/431
种属反应性
Human, Mouse
验证应用
WB
分子量
45 KDa
阳性对照
Osteoblast/chondrocyte specific.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-2000: 1:5000-10000
发表文章中的应用
| WB | 查看 13 篇文献如下 |
| IHC-P | 查看 6 篇文献如下 |
| IF | 查看 4 篇文献如下 |
发表文章中的种属
| Mouse | 查看 8 篇文献如下 |
| Rat | 查看 7 篇文献如下 |
| Human | 查看 4 篇文献如下 |
| Rabbit | 查看 1 篇文献如下 |
靶点
功能
Transcription factor Sp7, also called Osterix (Osx), is a protein that in humans is encoded by the SP7 gene. It is a member of the Sp family of zinc-finger transcription factors [5] It is highly conserved among bone-forming verterbrate species. It plays a major role, along with Runx2 and Dlx5 in driving the differentiation of mesenchymal precursor cells into osteoblasts and eventually osteocytes. Sp7 also plays a regulatory role by inhibiting chondrocyte differentiation maintaining the balance between differentiation of mesenchymal precursor cells into ossified bone or cartilage. Mutations of this gene have been associated with multiple dysfunctional bone phenotypes in vertebrates. During development, a mouse embryo model with Sp7 expression knocked out had no formation of bone tissue. Through the use of GWAS studies, the Sp7 locus in humans has been strongly associated with bone mass density. [10] In addition there is significant genetic evidence for its role in diseases such as Osteoge nesis imperfecta (OI). In a developing organism, Sp7 serves as one of the most important regulatory shepherds for bone formation. Outside of the context of development, in adult mice ablation of Sp7 led to a lack of new bone formation, highly irregular cartilage accumulation beneath the growth plate and defects in osteocyte maturation and functionality.
背景文献
暂无
亚细胞定位
Nucleus.
UNIPROT
别名
MGC126598 antibody
Osterix antibody
Osx antibody
Sp 7 antibody
SP7 antibody
Sp7 transcription factor antibody
SP7_HUMAN antibody
Transcription factor Sp7 antibody
Zinc finger protein osterix antibody
图片
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Sample:Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-Osterix(ER1914-47)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD -
Sample:
Lane 1: Mouse Embryo tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Primary: Anti-SP7/Osterix (ER1914-47) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Secreted Frizzled‐Related Protein 2 Promotes Osteogenic Differentiation and Bone Regeneration in Perthes Disease When Targeted by miR‐106a‐5p
Author: Song Yu
PMID: 40976879
期刊: Journal Of Cellular And Molecular Medicine
应用: WB
反应种属: Rabbit
发表时间: 2025 Sept
-
Citation
-
COPB1 deficiency triggers osteoporosis with elevated iron stores by inducing osteoblast ferroptosis
Author: Yike Wang, Ruizhi Zhang, Aifei Wang, Xiao Wang, Xiongyi Wang, Jiajun Zhang, Gongwen Liu, Kai Huang, Baoshan Liu, Yutong Hu, Sheng Pan, Xieyidai Ruze, Qiaocheng Zhai, Youjia Xu
PMID: 40206560
期刊: Journal of Orthopaedic Translation
应用: WB
反应种属: Mouse
发表时间: 2025 Mar
-
Citation
-
Kaempferol combats the osteogenic differentiation damage of periodontal ligament stem cells in periodontitis via regulating EphrinB2-mediated PI3K/Akt and P38 pathways
Author: Jiao Cao, Yue Li, Mengying Si, Shaoyang Ma, Meng Li, Anbang Shi, Jin Liu, Ang Li
PMID: 40220409
期刊: Phytomedicine
应用: WB
反应种属: Human
发表时间: 2025 Apr
-
Citation
-
Outer Membrane Vesicles Derived From Fusobacterium nucleatum Trigger Periodontitis Through Host Overimmunity
Author: Li Zhang, Demao Zhang, Chengcheng Liu, Boyu Tang, Yujia Cui, Daimo Guo, Mengmeng Duan, Ying Tu, Huiling Zheng, Xinjie Ning, Yang Liu, Haoran Chen, Minglei Huang, Zhixing Niu, Yanfang Zhao, Xiaoheng Liu, Jing Xie
PMID: 39475060
期刊: Advanced Science
应用: IF
反应种属: Rat
发表时间: 2024 Oct
-
Citation
-
A DNA tetrahedron-based ferroptosis-suppressing nanoparticle: superior delivery of curcumin and alleviation of diabetic osteoporosis
Author: Li Yong,et al
PMID: 38424439
期刊: Bone Research
应用: IF
反应种属: Mouse
发表时间: 2024 Mar
-
Citation
-
Atorvastatin exerts a preventive effect against steroid-induced necrosis of the femoral head by modulating Wnt5a release
Author: Wu Junfeng,et al
PMID: 38971901
期刊: Archives Of Toxicology
应用: IHC-P
反应种属: Rat
发表时间: 2024 Jul
-
Citation
-
Long non‐coding RNA LncTUG1 regulates favourable compression force‐induced cementocytes mineralization via PU. 1/TLR4/SphK1 signalling
Author: Wang Han,et al
PMID: 38318762
期刊: Cell Proliferation
应用: IHC-P
反应种属: Mouse
发表时间: 2024 Feb
-
Citation
-
β-catenin inhibition disrupts the homeostasis of osteogenic/adipogenic differentiation leading to the development of glucocorticoid-induced osteonecrosis of the femoral head
Author: Xia Chenjie,et al
PMID: 38376133
期刊: eLife
应用: WB
反应种属: Human,Mouse
发表时间: 2024 Feb
-
Citation
-
MicroRNA-29c-tetrahedral framework nucleic acids: Towards osteogenic differentiation of mesenchymal stem cells and bone regeneration in critical-sized calvarial defects
Author: Jiafei Sun, Xingyu Chen, Yunfeng Lin, Xiaoxiao Cai
PMID: 38414296
期刊: Cell Proliferation
应用: IF,WB
反应种属: Rat
发表时间: 2024 Feb
-
Citation
-
The effects of different forms of exercise during the early life on the bone microstructure of ovariectomized mice
Author: Yuxuan Wang,et al
PMID: 39645067
期刊: Bone
应用: WB
反应种属: Mouse
发表时间: 2024 Dec
-
Citation
-
Granulocyte colony-stimulating factor mediates bone loss via the activation of IL-1β/JNK signaling pathway in murine Staphylococcus aureus-induced osteomyelitis
Author: Song Mingrui,et al
PMID: 39163688
期刊: International Immunopharmacology
应用: IF
反应种属: Mouse
发表时间: 2024 Aug
-
Citation
-
Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3
Author: Xiao, X., Zou, S., & Chen, J.
PMID: 36890454
期刊: BMC Molecular and Cell Biology
应用: WB
反应种属: Mouse
发表时间: 2023 Mar
-
Citation
-
Effects of Puerarin‐Loaded Tetrahedral Framework Nucleic Acids on Osteonecrosis of the Femoral Head
Author:
PMID: 37317020
期刊: Small
应用: WB
反应种属: Rat
发表时间: 2023 Jun
-
Citation
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Glucocorticoid-induced activation of NOX/ROS/NF-κB signaling in MSCs contributes to the development of GONFH
Author: Xu, H., Zeng, Q., Zou, K., Huang, H., Chen, J., Wang, P., Yuan, W., Xiao, L., Tong, P., & Jin, H.
PMID: 37306805
期刊: Apoptosis
应用: WB,IHC-P
反应种属: Mouse
发表时间: 2023 Jun
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Citation
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Glycyrrhizic acid alters the hyperoxidative stress-induced differentiation commitment of MSCs by activating the Wnt/β-catenin pathway to prevent SONFH
Author: Xu, H., Fang, L., Zeng, Q., Chen, J., Ling, H., Xia, H., Ge, Q., Wu, C., Zou, K., Wang, X., Wang, P., Yuan, W., Dong, R., Hu, S., Xiao, L., He, B., Tong, P., & Jin, H.
PMID: 36541285
期刊: Food & Function
应用: IHC-P
反应种属: Rat
发表时间: 2023 Jan
-
Citation
-
Cyclic di-adenosine monophosphate regulates the osteogenic and adipogenic differentiation of hPDLSCs via MAPK and NF-κB signaling
Author: Chen, S., Wu, Z., He, Y., Zhu, L., Wang, J., Lin, H., Xie, J., Zhou, C., & Zou, S.
PMID: 36825442
期刊: Acta Biochimica Et Biophysica Sinica
应用: WB
反应种属: Human
发表时间: 2023 Feb
-
Citation
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Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis
Author: Wu, Z., He, Y., Chen, S., Zhu, L., Wang, J., Zhang, D., Xie, J., Zou, S., & Zhou, C.
PMID: 36068594
期刊: Stem Cell Research & Therapy
应用: WB,IHC-P
反应种属: Rat
发表时间: 2022 Sept
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Citation
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Differential expression profiling of microRNAs in human placenta-derived mesenchymal stem cells cocultured with grooved porous hydroxyapatite Scaffolds
Author:
PMID: 35180361
期刊: DNA and Cell Biology
应用: WB
反应种属: Human
发表时间: 2022 Mar
-
Citation
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Mechanosensitive Piezo1 in Periodontal Ligament Cells Promotes Alveolar Bone Remodeling During Orthodontic Tooth Movement
Author: Jiang, Y., Guan, Y., Lan, Y., Chen, S., Li, T., Zou, S., Hu, Z., & Ye, Q.
PMID: 34880779
期刊: Front Physiology
应用: IHC-P,WB
反应种属: Rat
发表时间: 2021 Nov
-
Citation
Alternative Products
Sp7 / Osterix Recombinant Rabbit Monoclonal Antibody [PSH07-29]
Application: WB,IHC-P,IF-Tissue,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated