Cullin-3 Rabbit Polyclonal Antibody
Catalog# ER1706-70
Cullin-3 Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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Mouse
概述
产品名称
Cullin-3 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human Cullin-3 aa 724-768.
种属反应性
Human, Mouse
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 89 kDa
阳性对照
SH-SY5Y, 293T, mouse testis tissue lysate, human kidney tissue, human placenta tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:500
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FC
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1:50-1:100
靶点
功能
Cullin 3 is a protein that in humans is encoded by the CUL3 gene. Cullin 3 protein belongs to the family of cullins which in mammals contains eight proteins (Cullin 1, Cullin 2, Cullin 3, Cullin 4A, Cullin 4B, Cullin 5, Cullin 7 and Cullin 9). Cullin proteins are an evolutionarily conserved family of proteins throughout yeast, plants and mammals. Cullin 3 is a component of Cullin-RING E3 ubiquitin ligases complexes (CRLs) which are involved in protein ubiquitylation and represent a part of ubiquitin–proteasome system (UPS). Added ubiquitin moieties to the lysine residue by CRLs then target the protein for the proteasomal degradation. Cullin-RING E3 ubiquitin ligases are involved in many cellular processes responsible for cell cycle regulation, stress response, protein trafficking, signal transduction, DNA replication, transcription, protein quality control, circadian clock and development. Deletion of CUL3 gene in mice causes embryonic lethality.
背景文献
1. Li X et al. CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. Autophagy. 2021 Dec
2. Wu J et al. Cullin-3: Renal and Vascular Mechanisms Regulating Blood Pressure. Curr Hypertens Rep. 2020 Aug
序列相似性
Belongs to the cullin family.
组织特异性
Brain, spermatozoa, and testis (at protein level). Widely expressed.
翻译后修饰
Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
亚细胞定位
Golgi apparatus. Nucleus.
别名
CUL 3 antibody
Cul-3 antibody
cul3 antibody
CUL3_HUMAN antibody
Cullin-3 antibody
Cullin3 antibody
KIAA0617 antibody
PHA2E antibody
图片
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Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: 293T
Lane 3: Mouse testis tissue -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-70) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of SH-SY5Y cells with Cullin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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Western blot analysis of Cullin-3 on mouse testis tissue lysates with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 89 kDa
Observed band size: 80 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-70) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Cullin 3 Recombinant Rabbit Monoclonal Antibody [JE37-29]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
