USP14 Recombinant Rabbit Monoclonal Antibody [JU30-49]
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Catalog# ET1706-37
USP14 Recombinant Rabbit Monoclonal Antibody [JU30-49]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Zebrafish
概述
产品名称
USP14 Recombinant Rabbit Monoclonal Antibody [JU30-49]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human USP14 aa 351-494 / 494.
种属反应性
Human, Zebrafish
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, FC
分子量
Predicted band size: 56 kDa
阳性对照
K562 cell lysate, Hela cell lysate, zebrafish lysates, A431, HUVEC, LOVO, human colon carcinoma tissue, human kidney tissue, mouse prostate tissue, rat epididymis tissue, Jurkat.
偶联
unconjugated
克隆号
JU30-49
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
发表文章中的应用
| WB | 查看 2 篇文献如下 |
发表文章中的种属
| Mouse | 查看 2 篇文献如下 |
| Human | 查看 1 篇文献如下 |
靶点
功能
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP14 (ubiquitin specific peptidase 14), also known as TGT (tRNA-guanine transglycosylase), is a cytoplasmic protein that belongs to the ubiquitin-specific processing family of deubiquitinating enzymes. Existing as a homodimer within the cell, USP14 functions to cleave ubiquitin residues from both ubiquitinylated proteins and ubiquitin-fused precursors, thereby saving these proteins from proteasomal degradation. In mice, defects or mutations in the gene encoding USP14 cause retarded growth or fetal death, indicating that USP14 plays a key role in early developmental processes. Multiple isoforms of USP14 are expressed due to alternative splicing events.
背景文献
1. Perry JW et al. Antiviral activity of a small molecule deubiquitinase inhibitor occurs via induction of the unfolded protein response. PLoS Pathog 8:e1002783 (2012).
2. Xu D et al. Phosphorylation and activation of ubiquitin-specific protease-14 by Akt regulates the ubiquitin-proteasome system. Elife 4:e10510 (2015).
序列相似性
Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
亚细胞定位
Cytoplasm. Cell membrane.
UNIPROT
别名
Deubiquitinating enzyme 14 antibody
TGT antibody
tRNA guanine transglycosylase 60 kD subunit antibody
tRNA guanine transglycosylase antibody
Ubiquitin carboxyl terminal hydrolase 14 antibody
Ubiquitin carboxyl-terminal hydrolase 14 antibody
Ubiquitin specific peptidase 14 antibody
Ubiquitin specific processing protease 14 antibody
Ubiquitin specific protease 14 antibody
Ubiquitin thiolesterase 14 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of USP14 on different lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/1,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si USP14 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 60 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of USP14 on different lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/500 dilution.
Lane 1: K562 cell lysate
Lane 2: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 60 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of USP14 on zebrafish lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 60 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A431 cells labeling USP14 with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of HUVEC cells labeling USP14 with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of LOVO cells labeling USP14 with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-37) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-37) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-37) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-37) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of USP14 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-37, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy
Author: Tang, C., Jin, M., Ma, B., Cao, B., Lin, C., Xu, S., Li, J., & Xu, Q.
PMID: 36653442
应用: WB
反应种属: Human,Mouse
发表时间: 2023 Jan
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Citation
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Yap1-Usp14 Axis Inhibits Neuronal Mitophagy During Neonatal Hypoxia–Ischemia Encephalopathy by Regulation of Beclin-1 Ubiquitination in Mouse
Author:
PMID: 37062801
应用: WB
反应种属: Mouse
发表时间: 2023 Aug
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Citation