UGP2 Rabbit Polyclonal Antibody
Catalog# R1404-5
UGP2 Rabbit Polyclonal Antibody
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WB
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Human
-
Mouse
-
Rat
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Zebrafish
概述
产品名称
UGP2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human UGP2 aa 60-120.
种属反应性
Human, Mouse, Rat, Zebrafish
验证应用
WB
分子量
Predicted band size: 57 kDa
阳性对照
Human liver, rat liver, mouse liver, human heart, NIH/3T3, HepG2
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
靶点
功能
UTP—glucose-1-phosphate uridylyltransferase also known as glucose-1-phosphate uridylyltransferase (or UDP–glucose pyrophosphorylase) is an enzyme associated with glycogenesis. It synthesizes UDP-glucose from glucose-1-phosphate and UTP. It plays a central role as a glucosyl donor in cellular metabolic pathways.
背景文献
1. "The importance of conserved residues in human liver UDPglucose pyrophosphorylase." Chang H.-Y., Peng H.-L., Chao Y.C., Duggleby R.G. Eur. J. Biochem. 236:723-728(1996)
2. "The crystal structure of human UDP-glucose pyrophosphorylase reveals a latch effect that influences enzymatic activity." Yu Q., Zheng X. Biochem. J. 442:283-291(2012)
序列相似性
Belongs to the UDPGP type 1 family.
亚细胞定位
Cytoplasm
UNIPROT
别名
pHC379 antibody
UDP glucose diphosphorylase antibody
UDP glucose pyrophosphorylase 1 antibody
UDP glucose pyrophosphorylase 2 antibody
UDP glucose pyrophosphorylase antibody
UDP-glucose pyrophosphorylase antibody
UDPG antibody
UDPGP 2 antibody
UDPGP antibody
UDPGP2 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of UGP2 on different lysates with Rabbit anti-UGP2 antibody (R1404-5) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-UGP2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57/56 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1404-5) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis on cell lysates using anti- UGP2 rabbit polyclonal antibodies.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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