Skip to content

HRP Conjugated GST Rabbit Polyclonal Antibody

RMB: 1200

产品规格

现在订购，【现货】

点击询价

联系我们

邮箱: sales@huabio.cn

电话: 0571-88062880

查找各地代理商

Catalog# 1006-3

HRP Conjugated GST Rabbit Polyclonal Antibody

Application

  • WB

  • ELISA

Reactivity

  • Species independent

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

HRP Conjugated GST Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

peptide

种属反应性

Species independent

验证应用

WB, ELISA

偶联

HRP

RRID

AB_3068723

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:5,000-1:10,000

  • ELISA

  • 1:10,000

发表文章中的应用

WB 查看 2 篇文献如下

发表文章中的种属

rice 查看 1 篇文献如下

靶点

功能

The pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots, as well as for immunogen affinity purification of proteins expressed in E. coli.

背景文献

暂无

别名

Glutathione S Transferase antibody

Glutathione S-transferase class-mu 26 kDa isozyme antibody

GST antibody

Sj26 antigen antibody

SjGST antibody

图片

  • Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (<a href="/products/1006-3" style="font-weight: bold;text-decoration: underline;">1006-3</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: 293T transfected with GST cell lysate<br />Lane 3: 293T transfected with GST-tagged Histone H3.1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/1006-3" style="font-weight: bold;text-decoration: underline;">1006-3</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (1006-3) at 1/5,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: 293T transfected with GST cell lysate
    Lane 3: 293T transfected with GST-tagged Histone H3.1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1006-3) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

搜索

主菜单

请您留言

杭州华安生物技术有限公司 0571-88062880

您需要的服务
抗体咨询与购买
提交