Stella Rabbit Polyclonal Antibody
Catalog# R1306-1
Stella Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
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Mouse
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Zebrafish
预测的种属支持售后
概述
产品名称
Stella Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within mouse Stella aa 101-150 / 150 (ID:Q8QZY).
种属反应性
Human, Mouse (Predicted: Zebrafish)
预测的种属支持售后
验证应用
WB, IHC-P
分子量
Predicted band size: 18 kDa
阳性对照
HeLa cell lysate, HEK-293 cell lysate, F9 cell lysate, NCCIT cell lysate, mouse teratoma tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:100-1:200
靶点
功能
Stella is a primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. It participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. It is important for the totipotent/pluripotent states continuing through preimplantation development.
背景文献
1. "Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma." Clark A.T., Rodriguez R.T., Bodnar M.S., Abeyta M.J., Cedars M.I., Turek P.J., Firpo M.T., Reijo Pera R.A. Stem Cells 22:169-179(2004)
2. "Dppa3 is a marker of pluripotency and has a human homologue that is expressed in germ cell tumours." Bowles J., Teasdale R.P., James K., Koopman P. Cytogenet. Genome Res. 101:261-265(2003)
组织特异性
Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
亚细胞定位
Cell nucleus, cytoplasm
别名
Compaction associated protein 1 antibody
Developmental pluripotency associated 3 antibody
Developmental pluripotency associated protein 3 antibody
Developmental pluripotency-associated protein 3 antibody
Dppa3 antibody
DPPA3_HUMAN antibody
PGC7 antibody
Stella related protein antibody
Stella-related protein antibody
图片
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Western blot analysis of Stella on different lysates with Rabbit anti-Stella antibody (R1306-1) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: F9 cell lysate
Lane 4: NCCIT cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 25 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1306-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse teratoma tissue using anti-Stella rabbit polyclonal antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"