Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]
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Catalog# ET7108-13
Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]
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WB
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IHC-P
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FC
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IP
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IF-Cell
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IF-Tissue
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Human
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Mouse
概述
产品名称
Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human Pumilio 1 aa 79-223 .
种属反应性
Human, Mouse
验证应用
WB, IHC-P, FC, IP, IF-Cell, IF-Tissue
分子量
Predicted band size: 126 kDa
阳性对照
SiHa cell lysate, 293T, SiHa, human colon tissue, human appendix tissue, mouse kidney tissue, mouse small intestine tissue.
偶联
unconjugated
克隆号
JG37-75
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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IP
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1:10-1:50
靶点
功能
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs.
背景文献
1. Lee S et al. Noncoding RNA NORAD regulates genomic stability by sequestering PUMILIO proteins. Cell 164:69-80 (2016).
2. Narita R et al. A novel function of human Pumilio proteins in cytoplasmic sensing of viral infection. PLoS Pathog 10:E1004417-E1004417 (2014).
组织特异性
Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.
翻译后修饰
Phosphorylation at Ser-714 promotes RNA-binding activity. Following growth factor stimulation phosphorylated at Ser-714, promoting binding to the 3'-UTR of CDKN1B/p27 mRNA.
亚细胞定位
Cytoplasm. Nucleus.
别名
HsPUM antibody
KIAA0099 antibody
PUM 1 antibody
PUM L1 antibody
Pum1 antibody
PUM1_HUMAN antibody
PUMH 1 antibody
PUMH antibody
PUMH1 antibody
pumilio homolog 1 (Drosophila) antibody
展开图片
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Western blot analysis of Pumilio 1 on SiHa cell lysate using anti-Pumilio 1 antibody at 1/1,000 dilution.
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ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of 293T cells with Pumilio 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"