EHMT2 / G9A Recombinant Rabbit Monoclonal Antibody [JE50-84]
Catalog# ET7110-08
EHMT2 / G9A Recombinant Rabbit Monoclonal Antibody [JE50-84]
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WB
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IHC-P
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Human
概述
产品名称
EHMT2 / G9A Recombinant Rabbit Monoclonal Antibody [JE50-84]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human G9A aa 1,060-1,210 / 1,210.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 132 kDa
阳性对照
Human colon carcinoma tissue, human breast carcinoma tissue, human stomach carcinoma tissue.
偶联
unconjugated
克隆号
JE50-84
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IHC-P
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1:50-1:400
靶点
功能
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
背景文献
1. Huang J. et. al. G9a and Glp methylate lysine 373 in the tumor suppressor p53. J. Biol. Chem. 285:9636-9641(2010).
2. Yu Y. et. al. Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA. Mol. Cell 46:7-17(2012).
序列相似性
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
组织特异性
Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
翻译后修饰
Methylated at Lys-185; automethylated.
亚细胞定位
Nucleus, chromosome.
UNIPROT
别名
Ankyrin repeat containing protein antibody
Bat 8 antibody
Bat8 antibody
C6orf30 antibody
DKFZp686H08213 antibody
EHMT 2 antibody
Ehmt2 antibody
EHMT2_HUMAN antibody
Euchromatic histone lysine methyltransferase 2 antibody
Euchromatic histone lysine N methyltransferase 2 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of EHMT2 / G9A on different lysates with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-EHMT2 / G9A KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 132 kDa
Observed band size: 160, 170 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-08) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-EHMT2 / G9A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-EHMT2 / G9A antibody (ET7110-08) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-08) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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