DAZL Recombinant Rabbit Monoclonal Antibody [JA10-36]
Catalog# ET1704-75
DAZL Recombinant Rabbit Monoclonal Antibody [JA10-36]
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WB
-
IHC-P
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Human
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Mouse
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Rat
概述
产品名称
DAZL Recombinant Rabbit Monoclonal Antibody [JA10-36]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within DAZL aa 10-59 / 295.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 33 kDa
阳性对照
Human testis tissue, rat testis tissue.
偶联
unconjugated
克隆号
JA10-36
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500
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IHC-P
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1:400
靶点
功能
DAZL (deleted in azoospermia-like), an RNA-binding protein that effects spermatogenesis, is an autosomal homolog of the Y chromosome DAZ (deleted in azoospermia). The gene encoding human DAZL maps to chromosome locus 3p24. DAZL, like other members of The DAZ family members are included in germ cells in both male and female gonads. A Thr 54-to-Ala mutation within the RNA-recognition domain of DAZL proteins contributes to spermatogenic failure. Infertility in DAZL knockout catisatalities are unable to complete the first meiotic prophase in the first wave of spermatogenesis.
背景文献
1. Wang H et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016).
2. Fleck D et al. Distinct purinergic signaling pathways in prepubescent mouse spermatogonia. J Gen Physiol 148:253-71 (2016).
序列相似性
Belongs to the RRM DAZ family.
组织特异性
Testis specific.
亚细胞定位
Cytoplasm. Nucleus. Predominantly cytoplasmic.
别名
DAZ homolog antibody
DAZ like autosomal antibody
DAZ-like autosomal antibody
DAZH antibody
DAZL 1 antibody
DAZL antibody
DAZL_HUMAN antibody
DAZL1 antibody
DAZLA antibody
Deleted in azoospermia like 1 antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DAZL antibody (ET1704-75) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-DAZL antibody (ET1704-75) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"