TAX1BP1 Recombinant Rabbit Monoclonal Antibody [JE34-80]
Catalog# HA721648
TAX1BP1 Recombinant Rabbit Monoclonal Antibody [JE34-80]
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
TAX1BP1 Recombinant Rabbit Monoclonal Antibody [JE34-80]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human TAX1BP1 aa 740-789 / 789.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 91 kDa
阳性对照
K-562 cell lysate, A549 cell lysate, 293T cell lysate, Jurkat cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse testis tissue, rat testis tissue.
偶联
unconjugated
克隆号
JE34-80
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IHC-P
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1:1,000
靶点
功能
Tax1-binding protein 1 is a protein that in humans is encoded by the TAX1BP1 gene. TAX1BP1 has been shown to interact with TNFAIP3 and TRAF6. TAX1BP1 binds to TNFAIP3 or TRAF6 and suppresses NF-kappaB transcriptional activation.
背景文献
1. White J et al. Multifaceted roles of TAX1BP1 in autophagy. Autophagy. 2023 Jan
2. Sarraf SA et al. Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain. Mol Cell. 2020 Dec
亚细胞定位
Cytoplasm, Mitochondrion, Preautophagosomal structure, Cytoplasmic vesicle, autophagosome.
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展开图片
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Western blot analysis of TAX1BP1 on different lysates with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/1,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: Mouse testis tissue lysate (40 µg/Lane)
Lane 6: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 91 kDa
Observed band size: 91 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721648) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of TAX1BP1 on different lysates with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-TAX1BP1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Exposure time: 180 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721648) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TAX1BP1 antibody (HA721648) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721648) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"