nNOS Recombinant Rabbit Monoclonal Antibody [ST520]
Catalog# ET1609-61
nNOS Recombinant Rabbit Monoclonal Antibody [ST520]
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WB
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
预测的种属支持售后
概述
产品名称
nNOS Recombinant Rabbit Monoclonal Antibody [ST520]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human nNOS aa 1398-1434 / 1434.
种属反应性
Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)
预测的种属支持售后
验证应用
WB, IF-Tissue, IHC-P, IP
分子量
Predicted band size: 161 kDa
阳性对照
Mouse brain tissue lysate, Mouse hippocampus tissue lysate, Mouse cerebellum tissue lysate, Rat hippocampus tissue lysate, Rat cerebellum tissue lysate, human skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.
偶联
unconjugated
克隆号
ST520
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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IP
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Use at an assay dependent concentration.
发表文章中的应用
| WB | 查看 2 篇文献如下 |
发表文章中的种属
| Mouse | 查看 2 篇文献如下 |
靶点
功能
Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.
背景文献
1. Khan M et al. Blocking a vicious cycle nNOS/peroxynitrite/AMPK by S-nitrosoglutathione: implication for stroke therapy. BMC Neurosci 16:42 (2015).
2. Li G et al. Firing of hippocampal neurogliaform cells induces suppression of synaptic inhibition. J Neurosci 34:1280-92 (2014).
序列相似性
Belongs to the NOS family.
组织特异性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
翻译后修饰
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
亚细胞定位
Cell membrane, sarcolemma, Cell projection, dendritic spine.
别名
2310005C01Rik antibody
BNOS antibody
Constitutive NOS antibody
EC 1.14.13.39 antibody
IHPS 1 antibody
IHPS1 antibody
N-NOS antibody
NC-NOS antibody
neuronal Nitric Oxide Synthase antibody
Neuronal NOS antibody
展开图片
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Western blot analysis of nNOS on different lysates with Rabbit anti-nNOS antibody (ET1609-61) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate (40 µg/Lane)
Lane 2: Mouse hippocampus tissue lysate (40 µg/Lane)
Lane 3: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 4: Rat hippocampus tissue lysate (40 µg/Lane)
Lane 5: Rat cerebellum tissue lysate (40 µg/Lane)
Predicted band size: 161 kDa
Observed band size: 161 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-61) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Integrated transcriptomics and metabolomics studies reveal the therapeutic effects of Astragalus polysaccharides on cancer cachexia muscle atrophy
Author: Tian Zhihan, Wang Yong, Liu Xue, Xin Xin, Liu Shuai, Qu Yiwei, Li Ziyuan, Su Xiaoyu, Ma Dufang
PMID: 40684040
期刊: Scientific Reports
应用: WB
反应种属: Mouse
发表时间: 2025 Jul
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Citation
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From Initiation to Maintenance: HIV-1 Gp120-induced Neuropathic Pain Exhibits Different Molecular Mechanisms in the Mouse Spinal Cord Via Bioinformatics Analysis Based on RNA Sequencing
Author:
PMID: 35059976
期刊: Journal Of Neuroimmune Pharmacology
应用: WB
反应种属: Mouse
发表时间: 2022 Dec
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Citation
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