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ACE2 Recombinant Rabbit Monoclonal Antibody [SN0754]

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Catalog# ET1611-58

ACE2 Recombinant Rabbit Monoclonal Antibody [SN0754]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IF-Tissue

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Hamster

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

ACE2 Recombinant Rabbit Monoclonal Antibody [SN0754]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human ACE2 aa 181-230 / 805.

种属反应性

Human, Mouse, Rat, Hamster

验证应用

WB, IF-Cell, IHC-P, IF-Tissue, IP

分子量

Predicted band size: 92 kDa

阳性对照

HepG2 cell lysate, 293T cell lysate, rat brain tissue lysate, rat kidney tissue lysate, human kidney tissue lysate, human small intestine tissue lysate, mouse kidney tissue lysate, mouse testis tissue lysate, hamster testis lysates, hamster stomach tissue lysates, 293, MCF-7, HepG2, human kidney tissue, human testis tissue, mouse kidney tissue.

偶联

unconjugated

克隆号

SN0754

RRID

AB_3070037

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:100-1:500

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

  • IP

  • Use at an assay dependent concentration.

发表文章中的应用

WB 查看 6 篇文献如下

靶点

功能

Angiotensin-converting enzyme (ACE) is a carboxyl-terminal dipeptidyl exopeptidase that converts angiotensin I to the potent vasopressive hormone, angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in blood pressure regulation and fluid/electrolyte homeostasis. ACE2 is the first known human homolog of ACE. Unlike ACE, which is expressed ubiquitously throughout the vasculature, ACE2 is expressed only in cardiac, renal and testicular cells.

背景文献

1. Thatcher SE et al. Angiotensin-Converting Enzyme 2 Decreases Formation and Severity of Angiotensin II-Induced Abdominal Aortic Aneurysms. Arterioscler Thromb Vasc Biol 34:2617-23 (2014).

2. Honorato-Sampaio K et al. Evidence that angiotensin-(1-7) is an intermediate of gonadotrophin-induced oocyte maturation in the rat preovulatory follicle. Exp Physiol 97:642-50 (2012).

序列相似性

Belongs to the peptidase M2 family.

组织特异性

Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level). Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in the renal proximal tubule and the small intestine (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

翻译后修饰

N-glycosylation on Asn-90 may limit SARS infectivity.; Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1.

亚细胞定位

Cell membrane, Cell projection, Cytoplasm, Membrane, Secreted.

UNIPROT

SwissProt: Q9BYF1 Human

SwissProt: Q8R0I0 Mouse

SwissProt: Q5EGZ1 Rat

SwissProt: A0A1U7QTA1 Hamster

别名

ACE 2 antibody

ACE related carboxypeptidase antibody

ACE-related carboxypeptidase antibody

ACE2 antibody

ACE2_HUMAN antibody

ACEH antibody

Angiotensin converting enzyme 2 antibody

Angiotensin converting enzyme homolog antibody

Angiotensin converting enzyme like protein antibody

Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody

展开

图片

  • Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat kidney tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 2 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat kidney tissue lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 100 kDa

    Exposure time: 2 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human kidney tissue lysate<br />Lane 2: Human small intestine tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 2 minutes;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/2,000 dilution.

    Lane 1: Human kidney tissue lysate
    Lane 2: Human small intestine tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 100 kDa

    Exposure time: 2 minutes;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse kidney tissue lysate<br />Lane 2: Mouse testis tissue lysate <br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

    Lane 1: Mouse kidney tissue lysate
    Lane 2: Mouse testis tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 100 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ACE2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE2 antibody at 1/1,000 dilution.

    Western blot analysis of ACE2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE2 antibody at 1/1,000 dilution.

  • ICC staining of ACE2 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of ACE2 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of ACE2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of ACE2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of ACE2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of ACE2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-58, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ACE2 antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ACE2 antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ACE2 antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-58) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution.<br /><br />Lane 1: Caco-2-si NT cell lysate<br />Lane 2: Caco-2-si ACE2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 120 kDa<br /><br />Exposure time: 1 minute 23 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-58" style="font-weight: bold;text-decoration: underline;">ET1611-58</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ACE2 on different lysates with Rabbit anti-ACE2 antibody (ET1611-58) at 1/1,000 dilution.

    Lane 1: Caco-2-si NT cell lysate
    Lane 2: Caco-2-si ACE2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 120 kDa

    Exposure time: 1 minute 23 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-58) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

同靶点 & 同通路的产品

metafield image

ACE2 Rabbit Polyclonal Antibody

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated

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