HRP Conjugated M13 Recombinant Mouse Monoclonal Antibody [PS00-01]
Catalog# HA601057
HRP Conjugated M13 Recombinant Mouse Monoclonal Antibody [PS00-01]
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ELISA
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M13 phage
概述
产品名称
HRP Conjugated M13 Recombinant Mouse Monoclonal Antibody [PS00-01]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
M13 Bacteriophage.
种属反应性
M13 phage
验证应用
ELISA
阳性对照
M13 Bacteriophage.
偶联
HRP
克隆号
PS00-01
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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ELISA
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1:4,000-1:30,000
靶点
功能
M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 50 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 2700 copies of p8 to make the coat about 900 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single stranded genome it packages. The general stages to a viral life cycle are: infection, replication of the viral genome, assembly of new viral particles and then release of the progeny particles from the host. Filamentous phage use a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single stranded DNA genome to a double stranded replicative form.
背景文献
1. Khalil, A.S. et al., Single M13 Bacteriophage Tethering and Stretching. Proc Natl Acad Sci. USA. 104 (12): 4892-7.
2. Sitohy, M. et al., Inhibition of Bacteriophage m13 Replication With Esterified Milk Proteins. J Agric Food Chem. 54 (11): 3800-6.
别名
Bacteriophage M13 antibody
Coliphage M13 antibody
Enterobacteria phage M13 antibody
M13 virus antibody
Phage M13 antibody
图片
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Direct ELISA analysis of M13 was performed by coating HSA/BSA (2 μg/mL in pH 9.6 carbonate buffer) in 96-well plate, and then add the indicated amounts of M13 Bacteriophages displaying anti-HSA Fab overnight at 4℃. Incubated with 100 µl per well of anti-M13 monoclonal antibody starting at a dilution of 1/1,000 and serially diluting it to a dilution of 1:64,000 for 1 hours at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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