ESD Recombinant Mouse Monoclonal Antibody [J0-8-R]
Catalog# HA601305
ESD Recombinant Mouse Monoclonal Antibody [J0-8-R]
-
WB
-
Human
-
Mouse
-
Rat
概述
产品名称
ESD Recombinant Mouse Monoclonal Antibody [J0-8-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human ESD aa 1-182 / 282.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 31 kDa
阳性对照
K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse colon tissue lysate, mouse stomach tissue lysate, rat kidney tissue lysate, rat colon tissue lysate, rat stomach tissue lysate.
偶联
unconjugated
克隆号
J0-8-R
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:20,000
靶点
功能
ESD (esterase D) is also known as S-formylglutathione hydrolase and is a 282 amino acid protein that is a member of the esterase D family. ESD is highly expressed in placenta, kidney, liver and erythrocytes, and is localized to the cytoplasm, as well as to cytoplasmic vesicles. The main function of ESD is to detoxify formaldehyde while providing energy. Formaldehyde is oxidized by ADH5 which yields S-formylglutathione. ESD then catalyzes the hydrolysis of S-formylglutathione to the reduced forms of formic acid and glutathione. In addition, ESD hydrolyzes a variety of different neutral ester substrates and can act as a carboxylesterase. ESD may also act as a cysteine hydrolase which is inactivated by thiol alkylating agents. ESD gene polymorphism can lead to reduced enzymatic activity which may cause susceptibility to many conditions, including toxic liver cirrhosis, retinoblastoma, obesity and autism.
背景文献
1. Li W et al. Esterase D enhances type I interferon signal transduction to suppress foot-and-mouth disease virus replication. Mol Immunol 75:112-21 (2016).
2. Kaźmierczak M et al. Esterase D and gamma 1 actin level might predict results of induction therapy in patients with acute myeloid leukemia without and with maturation. Med Oncol 30(4):725 (2013).
亚细胞定位
Cytoplasm. Cytoplasmic vesicle.
别名
EC 3.1.2.12 antibody
Es-10 antibody
Es10 antibody
ESD antibody
ESTD_HUMAN antibody
Esterase 10 antibody
Esterase D antibody
Esterase D formylglutathione hydrolase antibody
FGH antibody
FGH, included antibody
展开图片
-
Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 minute 13 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/2,000 dilution.
Lane 1: Human kidney tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Mouse colon tissue lysate
Lane 4: Mouse stomach tissue lysate
Lane 5: Rat kidney tissue lysate
Lane 6: Rat colon tissue lysate
Lane 7: Rat stomach tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 1 minute 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA601305) at 1/20,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ESD KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601305) at 1/20,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"