ASS1 Recombinant Mouse Monoclonal Antibody [11F1-RA]
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Catalog# HA601101
ASS1 Recombinant Mouse Monoclonal Antibody [11F1-RA]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
概述
产品名称
ASS1 Recombinant Mouse Monoclonal Antibody [11F1-RA]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within Human ASS1 aa 1-412 / 412.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Cell
分子量
Predicted band size: 47 kDa
阳性对照
HepG2 cell lysate, THP-1 cell lysate, SiHa cell lysate, rat kidney tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue, human kidney tissue, MCF-7.
偶联
unconjugated
克隆号
11F1-RA
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2b
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000-1:20,000
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IHC-P
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1:1,000
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IF-Cell
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1:200
靶点
功能
Argininosuccinate synthetase is an enzyme that in humans is encoded by the ASS1 gene. The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Two transcript variants encoding the same protein have been found for this gene. Mutations in the chromosome 9 copy of ASS cause citrullinemia. 40% to 90%[8] of bladder cancers are deficient in argininosuccinate synthetase.
背景文献
1. Carritt B, Goldfarb PS, Hooper ML, Slack C (Jun 1977). "Chromosome assignment of a human gene for argininosuccinate synthetase expression in Chinese hamsterxhuman somatic cell hybrids". Exp Cell Res. 106 (1): 71–8.
2. Beaudet AL, O\'Brien WE, Bock HG, Freytag SO, Su TS (Mar 1986). "The human argininosuccinate synthetase locus and citrullinemia". Adv Hum Genet. 15: 161–96, 291–2.
亚细胞定位
Cytoplasm
别名
Argininosuccinate synthase 1 antibody
Argininosuccinate synthase antibody
Argininosuccinate synthetase 1 antibody
ASS antibody
Ass-1 antibody
ass1 antibody
ASSA antibody
ASSY_HUMAN antibody
Citrulline aspartate ligase antibody
Citrulline--aspartate ligase antibody
展开图片
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Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA601101) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: THP-1 cell lysate (10 µg/Lane)
Lane 3: SiHa cell lysate (10 µg/Lane)
Lane 4: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601101) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA601101) at 1/20,000 dilution.
Lane 1: Mouse liver tissue lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (20 µg/Lane)
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601101) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA601101) at 1/2,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD ASS1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601101) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ASS1 antibody (HA601101) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601101) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-ASS1 antibody (HA601101) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601101) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of MCF-7 cells labeling ASS1 with Mouse anti-ASS1 antibody (HA601101) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-ASS1 antibody (HA601101) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"