Trop2 Recombinant Antibody [A5C6] - Human IgG1 (Chimeric)
Catalog# HA600012
Trop2 Recombinant Antibody [A5C6] - Human IgG1 (Chimeric)
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ELISA
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FC
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Human
概述
产品名称
Trop2 Recombinant Antibody [A5C6] - Human IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within Human TROP2 aa27-274 (Extracellular) in HEK293 cell.
种属反应性
Human
验证应用
ELISA, FC
分子量
35.7 kDa
阳性对照
Recombinant TROP2 protein, MDA-MB-468 cell.
偶联
unconjugated
克隆号
A5C6
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
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ELISA
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1:5,000-1:20,000
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FC
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1:100-1:200
靶点
功能
Tumor-associated calcium signal transducer 2, also known as Trop-2 and as epithelial glycoprotein-1 antigen (EGP-1), is a protein that in humans is encoded by the TACSTD2 gene. This intronless gene encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. This antigen is the target of sacituzumab govitecan, an antibody-drug conjugate. TROP-2 is related to epithelial cell adhesion molecule (EpCAM), also called TROP-1, gp40, and KSA. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells. This human monoclonal chimeric antibody has been engineered from the mouse monoclonal parent antibody. By necessity, some mouse sequence is retained as part of the variable domain. When multiplexing with other mouse-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
背景文献
1. Anthony Simms et al. TROP-2 expression in papillary thyroid carcinoma: Potential Diagnostic Utility. Diagn Cytopathol. 2016 Jan;44(1):26-31.
亚细胞定位
Membrane.
UNIPROT
别名
Cell surface glycoprotein Trop 2 antibody
Cell surface glycoprotein Trop-2 antibody
Cell surface glycoprotein Trop2 antibody
Epithelial glycoprotein 1 antibody
GA733 1 antibody
GA7331 antibody
M1S 1 antibody
M1S1 antibody
Membrane component chromosome 1 surface marker 1 antibody
Pancreatic carcinoma marker protein GA733 1 antibody
展开图片
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The binding activity of HA600012 with Recombinant TROP2 protein.
Immobilized Recombinant TROP2 protein at 1 ug/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600012) for 1 hour at 25℃. Donkey anti human IgG-AP at 1/1000 dilution was used as the secondary antibody. The EC50 of HA600012 is 5.574 ng/ml. -
Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-468 (Human breast adenocarcinoma cell line) cells labeling TROP2 with HA600012 at 1/200 dilution (red) compared with Purified human IgG, Isotype Control (black). Goat anti human IgG-FITC at 1/1000 dilution was used as the secondary antibody.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"