PFKP Rabbit Polyclonal Antibody
Catalog# HA500472
PFKP Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
PFKP Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within human PFKP aa 601-784 / 784.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
86 kDa
阳性对照
A549 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, human prostate carcinoma tissue, mouse brain tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IHC-P
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1:50-1:200
发表文章中的应用
| WB | 查看 1 篇文献如下 |
发表文章中的种属
| Human | 查看 1 篇文献如下 |
靶点
功能
Phosphofructokinase, platelet, also known as PFKP is an enzyme which in humans is encoded by the PFKP gene. The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose 6-phosphate to fructose 1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM) and liver (PFKL) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Full tetrameric phosphofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.
背景文献
1. Shen J. et. al. PFKP is highly expressed in lung cancer and regulates glucose metabolism. Cell Oncol (Dordr). 2020 Aug
2. Lang L. et. al. PFKP Signaling at a Glance: An Emerging Mediator of Cancer Cell Metabolism. Adv Exp Med Biol. 2019
亚细胞定位
Cytoplasm.
别名
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6-phosphofructokinase type C antibody
9330125N24Rik antibody
FLJ40226 antibody
K6PP antibody
K6PP_HUMAN antibody
MGC105718 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of PFKP on different lysates with Rabbit anti-PFKP antibody (HA500472) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PFKP KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 86 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500472) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PFKP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500472, 1/5,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Mouse lung tissue lysate
Lane 3: Rat lung tissue lysate -
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-PFKP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500472, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PFKP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500472, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
