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Ly-6G Rabbit Polyclonal Antibody

RMB: 1500

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Catalog# 0809-11

Ly-6G Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Ly-6G Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within mouse Ly-6G aa 1-50 / 134.

种属反应性

Mouse

验证应用

WB

分子量

Predicted band size: 14 kDa

阳性对照

Mouse spleen tissue lysate, Mouse lung tissue lysate.

偶联

unconjugated

RRID

AB_3068683

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:2,000

发表文章中的种属

Mouse 查看 7 篇文献如下

靶点

功能

Ly-6G is a marker of myeloid differentiation, which is expressed on majority of myeloid cells in the bone marrow and granulocytes in the periphery. Ly-6G belongs to the Ly-6 family of glycosyl-phosphatidylinositol (GPI)-linked proteins. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation

背景文献

1. Fleming T.J., O\'Huigin C., Malek T.R.;"Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins.";J. Immunol. 150:5379 -5390(1993).

2. Tsou, C.L., Peters, W., Si, Y., Slaymaker, S., Aslanian, A.M., Weisberg, S.P.,Mack, M. and Charo, I.F. “Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites.” J. Clin. Invest. 117: 902-909(2007).

组织特异性

Expressed in bone marrow.

亚细胞定位

Cell membrane.

UNIPROT

SwissProt: P35461 Mouse

别名

Gr-1 antibody

Gr1 antibody

Ly-6G.1 antibody

Ly6G antibody

Lymphocyte antigen 6 complex locus G antibody

Lymphocyte antigen 6G antibody

图片

  • Western blot analysis of Ly-6G on different lysates with Rabbit anti-Ly-6G antibody (<a href="/products/0809-11" style="font-weight: bold;text-decoration: underline;">0809-11</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse lung tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 25~30 kDa<br /><br />Exposure time: 7 seconds;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/0809-11" style="font-weight: bold;text-decoration: underline;">0809-11</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ly-6G on different lysates with Rabbit anti-Ly-6G antibody (0809-11) at 1/1,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse lung tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 25~30 kDa

    Exposure time: 7 seconds;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0809-11) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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  1. IHC
  2. WB
  3. IF
  4. IF-Tissue
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