Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein
Catalog# HA210128
Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein
Product Information
Product name
Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein
Catalog No.
HA210128
Predicted molecular mass
The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result.
Protein construction description
Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe42-Tyr995(ITGA5) acidic tail and Gln21-Asp728(ITGB1) basic tail.
Accession
P05556-1(ITGB1)
Source
HEK293
Purity
> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC
Endotoxin
Less than 1EU per μg by the LAL method.
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Species
Human
Background
Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit.Alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis.
Usage guide
Storage
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
