gamma tubulin Mouse Monoclonal Antibody [10-A4]
Catalog# M1701-13
gamma tubulin Mouse Monoclonal Antibody [10-A4]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
概述
产品名称
gamma tubulin Mouse Monoclonal Antibody [10-A4]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human gamma tubulin aa 402-451 / 451.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Cell
分子量
Predicted band size: 51 kDa
阳性对照
Rat epididymis tissue, human tonsil tissue, human colon cancer tissue, mouse testis tissue.
偶联
unconjugated
克隆号
10-A4
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2a
纯化方式
Protein G affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:50-1:200
靶点
功能
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
背景文献
1. Morris EJ et al. Stat3 regulates centrosome clustering in cancer cells via Stathmin/PLK1. Nat Commun 8:15289 (2017).
2. Goldspink DA et al. Ninein is essential for apico-basal microtubule formation and CLIP-170 facilitates its
序列相似性
Belongs to the tubulin family.
翻译后修饰
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
亚细胞定位
Cytoplasm, cytoskeleton, centrosome.
别名
Gamma 1 tubulin antibody
Gamma 2 tubulin antibody
Gamma Tubulin 1 antibody
Gamma Tubulin 2 antibody
Gamma tubulin complex component 1 antibody
Gamma-2-tubulin antibody
GCP 1 antibody
GCP1 antibody
MGC131994 antibody
TBG2_HUMAN antibody
展开图片
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Western blot analysis of gamma tubulin on different lysates using anti-gamma tubulin antibody at 1/2,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: Human fetal brain tissue -
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.
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Immunocytochemistry analysis of Hela cells labeling gamma tubulin with Mouse anti-gamma tubulin antibody (M1701-13) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at RT, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-gamma tubulin antibody (M1701-13) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"