c-Maf Recombinant Rabbit Monoclonal Antibody [JE39-57]
Catalog# HA721378
c-Maf Recombinant Rabbit Monoclonal Antibody [JE39-57]
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WB
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Human
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Mouse
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Rat
概述
产品名称
c-Maf Recombinant Rabbit Monoclonal Antibody [JE39-57]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human c-Maf aa 324-373 / 373.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 38 kDa
阳性对照
A549 cell lysate, Raji cell lysate, mouse lung tissue lysate, mouse kidney tissue lysate, rat spleen tissue lysate, rat kidney tissue lysate.
偶联
unconjugated
克隆号
JE39-57
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
靶点
功能
Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.
背景文献
1. Eychene A et al. A new MAFia in cancer. Nat. Rev. Cancer 8:683-693 (2008)
2. Pouponnot C et al. Cell context reveals a dual role for Maf in oncogenesis. Oncogene 25:1299-1310 (2006)
亚细胞定位
Nucleus.
别名
AS42 oncogene homolog antibody
Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene antibody
Avian musculoaponeurotic fibrosarcoma (v maf) antibody
c maf proto oncogene antibody
cMaf antibody
maf antibody
MAF_HUMAN antibody
MAF2 antibody
MGC71685 antibody
Proto oncogene c Maf antibody
展开图片
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Western blot analysis of c-Maf on different lysates with Rabbit anti-c-Maf antibody (HA721378) at 1/1,000 dilution.
Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: Raji cell lysate (10 µg/Lane)
Lane 3: Mouse lung tissue lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lane 5: Rat spleen tissue lysate (20 µg/Lane)
Lane 6: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721378) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"