Sulfite oxidase Recombinant Rabbit Monoclonal Antibody [JE56-74]
Catalog# HA720008
Sulfite oxidase Recombinant Rabbit Monoclonal Antibody [JE56-74]
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WB
-
IHC-P
-
FC
-
Human
-
Mouse
概述
产品名称
Sulfite oxidase Recombinant Rabbit Monoclonal Antibody [JE56-74]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Sulfite oxidase aa 100-200.
种属反应性
Human, Mouse
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 60 kDa
阳性对照
293T cell lysate, Mouse liver tissue lysate, human kidney tissue, HepG2.
偶联
unconjugated
克隆号
JE56-74
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:500-1:1,000
靶点
功能
Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins.
背景文献
1. Nakamura K. et. al. SUOX is negatively associated with multistep carcinogenesis and proliferation in oral squamous cell carcinoma. Med Mol Morphol. 2018 Jun
2. Jin GZ. et. al. SUOX is a promising diagnostic and prognostic biomarker for hepatocellular carcinoma. J Hepatol. 2013 Sep
亚细胞定位
Mitochondrion intermembrane space.
别名
EC 1.8.3.1 antibody
mitochondrial antibody
Sulfite oxidase antibody
Sulfite oxidase mitochondrial antibody
Sulfite oxidase, mitochondrial precursor antibody
Suox antibody
SUOX_HUMAN antibody
图片
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☑ Knockdown (KD)
Western blot analysis of Sulfite oxidase on different lysates with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Sulfite oxidase KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 60 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720008) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Sulfite oxidase on different lysates with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/5,000 dilution.
Lane 1: 293T cell lysate
Lane 2: Mouse liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720008) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Sulfite oxidase antibody (HA720008) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720008) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Sulfite oxidase was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720008, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"