PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]
Catalog# HA721087
PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]
-
WB
-
IHC-P
-
Human
概述
产品名称
PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human PNKP aa 460-509/521.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 57 kDa
阳性对照
HepG2 cell lysate, 293 cell lysate, PC-3 cell lysate, Jurkat cell lysate, human colon carcinoma tissue.
偶联
unconjugated
克隆号
JE64-85
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500
-
IHC-P
-
1:100
靶点
功能
Bifunctional polynucleotide phosphatase/kinase is an enzyme that in humans is encoded by the PNKP gene. A detailed structural study of the crystallized mouse protein examined both the 5´-polynucleotide kinase and 3’-polynucleotide phosphatase activities. Additional features of the peptide sequence include a forkhead association (FHA) domain, ATP binding site and nuclear and mitochondrial localization sequences. PNKP has been shown to interact with DNA polymerase beta and XRCC1. The human gene encoding PNKP was observed to be mutated in patients with microcephaly, seizures and defects in DNA repair. A type of recessive ataxia is also associated with PNKP mutations. There are also newly characterized pathological variants of PNKP. Model organisms such as mice and Drosophila have been used to generate further insights.
背景文献
1. Bermúdez-Guzmán L. et. al. Mutational survivorship bias: The case of PNKP. PLoS One. 2020 Dec
2. Mani RS. et. al. Domain analysis of PNKP-XRCC1 interactions: Influence of genetic variants of XRCC1. J Biol Chem. 2019 Jan
亚细胞定位
Nucleus.
UNIPROT
别名
2''(3'')-polynucleotidase antibody
2'(3')-polynucleotidase antibody
Bifunctional polynucleotide phosphatase/kinase antibody
DEM 1 antibody
DEM1 antibody
DNA 5' kinase/3' phosphatase antibody
DNA 5''-kinase/3''-phosphatase antibody
EIEE10 antibody
Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
MCSZ antibody
展开图片
-
Western blot analysis of PNKP on different lysates with Rabbit anti-PNKP antibody (HA721087) at 1/500 dilution.
Lane 1: HepG2 cell lysate
Lane 2: 293 cell lysate
Lane 3: PC-3 cell lysate
Lane 4: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 60 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721087) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PNKP antibody (HA721087) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721087) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"