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PDIA3 / ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44]

RMB: 900 特惠 1500

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Catalog# ET1703-68

PDIA3 / ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44]

Application

  • WB

  • IP

  • IHC-P

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

PDIA3 / ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human PDIA3 aa 175-208 / 505.

种属反应性

Human

验证应用

WB, IP, IHC-P

分子量

Predicted band size: 57 kDa

阳性对照

HepG2 cell lysate, LNCaP cell lysate, human kidney tissue, human pancreas tissue, human uterus tissue.

偶联

unconjugated

克隆号

JM11-44

RRID

AB_3070423

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

发表文章中的应用

WB 查看 1 篇文献如下

发表文章中的种属

Human 查看 1 篇文献如下

靶点

功能

Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically-derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. MHC class I molecules assemble in the endoplasmic reticulum with chaperones before binding to the transporter associated with antigen processing (TAP). ERp57, also designated GRP57, GRP58, ERp60 and ERp61, is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. The human ERp57 gene maps to chromosome 15q15 and encodes a 505 amino acid protein. ERp57 has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved among the mammals. ERp57 may act as a protease, a protein disulfide isomerase, a phospholipase or a combination of these.

背景文献

1. He Y et al. Largescale Transcriptomics Analysis Suggests Over-Expression of BGH3, MMP9 and PDIA3 in Oral Squamous Cell Carcinoma. PLoS One 11:e0146530 (2016).

2. Kodavanti PR et al. Developmental exposure to a commercial PBDE mixture: effects on protein networks in the cerebellum and hippocampus of rats. Environ Health Perspect 123:428-36 (2015).

序列相似性

Belongs to the protein disulfide isomerase family.

组织特异性

Detected in the flagellum and head region of spermatozoa (at protein level).

亚细胞定位

Endoplasmic reticulum lumen. Melanosome.

UNIPROT

SwissProt: P30101 Human

别名

58 kDa glucose regulated protein antibody

58 kDa glucose-regulated protein antibody

58 kDa microsomal protein antibody

Disulfide isomerase ER 60 antibody

Disulfide isomerase ER-60 antibody

Endoplasmic reticulum resident protein 57 antibody

Endoplasmic reticulum resident protein 60 antibody

ER p57 antibody

ER protein 57 antibody

ER protein 60 antibody

展开

图片

  • Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (<a href="/products/ET1703-68" style="font-weight: bold;text-decoration: underline;">ET1703-68</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: LNCaP cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 57 kDa<br /><br />Exposure time: 15 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-68" style="font-weight: bold;text-decoration: underline;">ET1703-68</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (ET1703-68) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: LNCaP cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

    Exposure time: 15 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-68) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (<a href="/products/ET1703-68" style="font-weight: bold;text-decoration: underline;">ET1703-68</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-PDIA3 / ERp57 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 21 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-68" style="font-weight: bold;text-decoration: underline;">ET1703-68</a>) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (ET1703-68) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-PDIA3 / ERp57 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 50 kDa

    Exposure time: 21 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-68) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB
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