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Optineurin Rabbit Polyclonal Antibody

RMB: 900 特惠 1500

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Catalog# ER2001-02

Optineurin Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Optineurin Rabbit Polyclonal Antibody

抗体类型

Rabbit Polyclonal Antibody

免疫原

Synthetic peptide within human optineurin aa 1-40.

种属反应性

Human

验证应用

WB, FC

分子量

66 kDa

阳性对照

A431 cell lysates, human liver tissue, human kidney tissue, human placenta tissue, MCF-7.

偶联

unconjugated

RRID

AB_3069484

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Immunogen affinity purified.

应用稀释度

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

发表文章中的应用

WB 查看 1 篇文献如下

发表文章中的种属

Mouse 查看 1 篇文献如下

靶点

功能

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

背景文献

1. Structural insights into the ubiquitin recognition by OPTN (optineurin) and its regulation by TBK1-mediated phosphorylation. Li F. et. al. Autophagy. 2018

2. Phosphorylation of OPTN by TBK1 enhances its binding to Ub chains and promotes selective autophagy of damaged mitochondria. Richter B. et. al. Proc Natl Acad Sci U S A. 2016 Apr

亚细胞定位

Golgi apparatus, trans-Golgi network, Recycling endosome, perinuclear region, autophagosome, Cytoplasmic vesicle.

UNIPROT

SwissProt: Q96CV9 Human

别名

14.7K interacting protein antibody

Ag9 C5 antibody

ALS12 antibody

E3 14.7K interacting protein antibody

E3-14.7K-interacting protein antibody

FIP 2 antibody

FIP-2 antibody

FIP2 antibody

Glaucoma 1 open angle E (adult onset) antibody

Glaucoma 1 open angle E antibody

展开

图片

  • Western blot analysis of Optineurin on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-02" style="font-weight: bold;text-decoration: underline;">ER2001-02</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Optineurin on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-02, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of Optineurin was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER2001-02" style="font-weight: bold;text-decoration: underline;">ER2001-02</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Optineurin was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

Filter:
  1. WB

同靶点 & 同通路的产品

metafield image

Optineurin Rabbit Polyclonal Antibody

Application: WB,IHC-P,IF-Tissue

Reactivity: Mouse

Conjugate: unconjugated

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