Ly6g Rabbit Polyclonal Antibody
Catalog# 0809-11
Ly6g Rabbit Polyclonal Antibody
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WB
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FC
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Mouse
概述
产品名称
Ly6g Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant protein within mouse Ly6g aa 1-105.
种属反应性
Mouse
验证应用
WB, FC
分子量
Predicted band size: 14 kDa
阳性对照
Mouse spleen tissue lysate, Mouse lung tissue lysate.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000-1:5,000
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FC
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1:1,000
发表文章中的应用
| IHC-P | 查看 5 篇文献如下 |
| WB | 查看 1 篇文献如下 |
| IF | 查看 1 篇文献如下 |
| IHC | 查看 1 篇文献如下 |
| IF-tissue | 查看 1 篇文献如下 |
发表文章中的种属
| Mouse | 查看 9 篇文献如下 |
靶点
功能
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
背景文献
1. Ruscitti C et al. Recruited atypical Ly6G(+) macrophages license alveolar regeneration after lung injury. Sci Immunol. 2024 Aug
2. Iliakis CS et al. Never trust a single myeloid marker: Ly6G on repair-promoting lung macrophages. Sci Immunol. 2024 Aug
组织特异性
Expressed in bone marrow.
亚细胞定位
Cell membrane.
UNIPROT
别名
Gr-1 antibody
Gr1 antibody
Ly-6G.1 antibody
Ly6G antibody
Lymphocyte antigen 6 complex locus G antibody
Lymphocyte antigen 6G antibody
图片
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Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (0809-11) at 1/1,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 25~30 kDa
Exposure time: 7 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0809-11) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Ly6g on different lysates with Rabbit anti-Ly6g antibody (0809-11) at 1/5,000 dilution.
Lane 1: 293T transfected with empty control cell lysate (negative)
Lane 2: 293T transfected with Ly6g cell lysate (positive)
Lane 3: 293T transfected with Ly6f cell lysate (negative)
Lane 4: 293T transfected with Ly6i cell lysate (negative)
Lane 5: 293T transfected with Ly6c1 cell lysate (negative)
Lane 6: 293T transfected with Ly6c2 cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0809-11) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Liquiritin mitigates lower extremity deep vein thrombosis by inhibiting inflammation and oxidative stress via the NF-κB signaling pathway
Author: Zhang Jiacheng, Wang Nan, Xin Tianyou, Zhu Xiaojun, Lang Shengkun, Ge Xin
PMID: 40394684
期刊: Thrombosis Journal
应用: IHC
反应种属: Mouse
发表时间: 2025 May
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Citation
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GI-Y2, a novel gasdermin D inhibitor, attenuates sepsis-induced myocardial dysfunction by inhibiting gasdermin D-mediated pyroptosis in macrophages
Author: Yiling Mei, Xudong Chen, Si Shi, Wante Lin, Zhenfeng Cheng, Xiaoxi Fan, Wenqi Wu, Jibo Han, Weijian Huang, Bozhi Ye, Shanshan Dai
PMID: 40165368
期刊: British Journal Of Pharmacology
应用: IHC-P
反应种属: Mouse
发表时间: 2025 Mar
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Citation
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High-dose vitamin C attenuates radiation-induced pulmonary fibrosis by targeting S100A8 and S100A9
Author: Ma Li,et al
PMID: 39025374
期刊: Biochimica Et Biophysica Acta-Molecular Basis Of Disease
应用: IF
反应种属: Mouse
发表时间: 2024 Jul
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Citation
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Ozone-delivering nanocomposite hydrogel for acute Staphylococcus aureus osteomyelitis treatment via neutrophil regulation
Author: Wu Hangtian,et al
PMID: no pmid0510
期刊: Chemical Engineering Journal
应用: IHC-P
反应种属: Mouse
发表时间: 2024 Apr
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Citation
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FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation
Author: Tian T, Xie X, Yi W, et al
PMID: 37938970
期刊: Cell Reports
应用: IHC-P
反应种属: Mouse
发表时间: 2023 Nov
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Citation
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Integrated Bioinformatics Analysis and Experimental Verification of Immune Cell Infiltration and the Related Core Genes in Ulcerative Colitis
Author:
PMID: 37383675
期刊: Pharmacogenomics & Personalized Medicine
应用: IHC-P
反应种属: Mouse
发表时间: 2023 Jun
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Citation
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Syndecan-1 Shedding by Matrix Metalloproteinase-9 Signaling Regulates Alveolar Epithelial Tight Junction in Lipopolysaccharide-Induced Early Acute Lung Injury
Author: Zhang, D., Zhang, J. T., Pan, Y., Liu, X. F., Xu, J. W., Cui, W. J., Qiao, X. R., & Dong, L.
PMID: 34764672
期刊: Journal Of Inflammation Research
应用: WB
反应种属: Mouse
发表时间: 2021 Nov
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Citation
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Nanodefensin-encased hydrogel with dual bactericidal and pro-regenerative functions for advanced wound therapy
Author: Luo, G., Sun, Y., Zhang, J., Xu, Z., Lu, W., Wang, H., Zhang, Y., Li, H., Mao, Z., Ye, S., Cheng, B., & Fang, X.
PMID: 33664853
期刊: Theranostics
应用: IHC-P
反应种属: Mouse
发表时间: 2021 Jan
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Citation
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HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-κB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function
Author:
PMID: 33488933
期刊: Oxidative Medicine And Cellular Longevity
应用: IF-tissue
反应种属: Mouse
发表时间: 2020 Dec
-
Citation
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