KMT5A / SETD8 / Pr-SET7 Recombinant Rabbit Monoclonal Antibody [JM73-43]
Catalog# ET1705-59
KMT5A / SETD8 / Pr-SET7 Recombinant Rabbit Monoclonal Antibody [JM73-43]
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WB
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FC
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Human
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Mouse
概述
产品名称
KMT5A / SETD8 / Pr-SET7 Recombinant Rabbit Monoclonal Antibody [JM73-43]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human KMT5A aa 51-100 / 393.
种属反应性
Human, Mouse
验证应用
WB, FC
分子量
Predicted band size: 43 kDa
阳性对照
Mouse kidney tissue lysates, Mouse liver tissue lysates, 293T cell lysates, 293 cell lysates, 293T cells.
偶联
unconjugated
克隆号
JM73-43
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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FC
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1:50-1:100
靶点
功能
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. All monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions , benef playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contribute to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53 / TP53 at 'Lys-382', leading to repress p53 / TP53-target genes.
背景文献
1. Gursoy-Yuzugullu O et al. Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity. Oncotarget 8:24518-24532 (2017).
2. Zhou HR et al. Relationship between epigenetic changes in Wnt antagonists and acute leukemia. Oncol Rep 37:2663-2671 (2017).
序列相似性
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.
翻译后修饰
Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity.; Ubiquitinated and degraded by the DCX(DTL) complex.
亚细胞定位
Nucleus.
别名
H4 K20 HMTase antibody
H4 K20 specific histone methyltransferase antibody
H4-K20-HMTase SETD8 antibody
Histone H4 K20 methyltransferase antibody
Histone lysine N methyltransferase H4 lysine 20 specific antibody
Histone-lysine N-methyltransferase SETD8 antibody
Lysine N-methyltransferase 5A antibody
N-lysine methyltransferase SETD8 antibody
Pr SET 7 antibody
Pr SET7 antibody
展开图片
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Western blot analysis of KMT5A on different lysates using anti-KMT5A antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse kidney tissue lysates
Lane 2: Mouse liver tissue lysates
Lane 3: 293T cell lysates
Lane 4: 293 cell lysates -
Flow cytometric analysis of 293T cells with KMT5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"