Human TSLP Recombinant Rabbit Monoclonal Antibody [PSH12-89] - BSA and Azide free (Capture)
概述
产品名称
Human TSLP Recombinant Rabbit Monoclonal Antibody [PSH12-89] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human TSLP aa 29-159 (HA210952).
种属反应性
Human
验证应用
ELISA(Cap)
阳性对照
Recombinant Human TSLP protein (HA210952).
偶联
unconjugated
克隆号
PSH12-89
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH12-90] to Human TSLP antibody (Detector) (HA723491) and recombinant Human TSLP protein (HA210952) as the standard. The reference range value is 15.6-2,000 pg/mL.
靶点
功能
This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants.
背景文献
1. O\'Byrne PM et al. Development of an inhaled anti-TSLP therapy for asthma. Pulm Pharmacol Ther. 2023 Feb
2. Nakajima S et al. Anti-TSLP antibodies: Targeting a master regulator of type 2 immune responses. Allergol Int. 2020 Apr
亚细胞定位
Secreted.
UNIPROT
别名
Thymic stromal lymphopoietin antibody
Thymic stromal lymphopoietin protein TSLP antibody
Tslp antibody
TSLP protein antibody
TSLP_HUMAN antibody
图片
-
![Sandwich ELISA analysis of Human TSLP matched pair antibodies<br /><br />Capture: <a href="/products/HA723490" style="font-weight: bold;text-decoration: underline;">HA723490</a>, Human TSLP Rabbit mAb [PSH12-89]<br />Detector: <a href="/products/HA723491" style="font-weight: bold;text-decoration: underline;">HA723491</a>, Human TSLP Rabbit mAb [PSH12-90]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (<a href="/products/HA723490" style="font-weight: bold;text-decoration: underline;">HA723490</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (<a href="/products/HA210952" style="font-weight: bold;text-decoration: underline;">HA210952</a>) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723491" style="font-weight: bold;text-decoration: underline;">HA723491</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723490_1.png?v=20250416085759)
Sandwich ELISA analysis of Human TSLP matched pair antibodies
Capture: HA723490, Human TSLP Rabbit mAb [PSH12-89]
Detector: HA723491, Human TSLP Rabbit mAb [PSH12-90]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723490) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TSLP protein (HA210952) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723491, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of spiked TSLP in human cell culture media samples.
The concentrations of TSLPwere interpolated from the TSLP standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 1 25%, cell culture media 2 50%.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"