HLA-B Mouse Monoclonal Antibody [B10-G7]
Catalog# EM1707-31
HLA-B Mouse Monoclonal Antibody [B10-G7]
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WB
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IF-Cell
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IHC-P
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FC
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Human
概述
产品名称
HLA-B Mouse Monoclonal Antibody [B10-G7]
抗体类型
Mouse Monoclonal Antibody
免疫原
Purified recombinant fragment of human HLA-B (AA: 241-362) expressed in E. Coli.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P, FC
分子量
41 kDa
阳性对照
Human HLA-B recombinant protein, HLA-B-hIgGFc transfected HEK293, Ramos, A431, Hela, MCF-7, human ovarian cancer, human bladder cancer.
偶联
unconjugated
克隆号
B10-G7
产品特性
形态
Liquid
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:200
靶点
功能
Major histocompatibility complex (MHC) molecules form an integral part of the immune response system. They are cell-surface receptors that bind peptides and present them to T lymphocytes. Human leukocyte antigens (HLAs) are polymorphic members of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens: class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. HLA-B and HLA-C encode membrane anchored heavy chains which heterodimerize with a light chain (β-2-Microglobulin) to form MHC-I. Polymorphisms yield hundreds of HLA-B and HLA-C alleles.
背景文献
1. Lee HL et al.Identification of a novel HLA-B allele, HLA-B*40:238, in a Taiwanese individual.HLA, doi: 10.1111 (2017).
2. Yerly D et al.Structural Elements Recognized by Abacavir-Induced T Cells. Int J Mol Sci 18:7(2017).
亚细胞定位
Membrane
UNIPROT
别名
Antigen Presenting Molecule antibody
Cw*1701 antibody
D6S204 antibody
FLJ27082 antibody
HLA A antibody
HLA B antibody
HLA C antibody
HLA class 1 A antibody
HLA class 1 B antibody
HLA class 1 C antibody
展开图片
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Western blot analysis of HLA-B on human HLA-B recombinant protein using anti-HLA-B antibody at 1/1,000 dilution.
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Western blot analysis of HLA-B on HEK293 (1) and HLA-B-hIgGFc transfected HEK293 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.
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Western blot analysis of HLA-B on Ramos(1) and A431 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.
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ICC staining HLA-B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of MCF-7 cells with HLA-B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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