Follistatin Recombinant Rabbit Monoclonal Antibody [JE31-78]
Catalog# HA722532
Follistatin Recombinant Rabbit Monoclonal Antibody [JE31-78]
-
WB
-
IHC-P
-
Mouse
-
Rat
-
Human
预测的种属支持售后
概述
产品名称
Follistatin Recombinant Rabbit Monoclonal Antibody [JE31-78]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human Follistatin aa 1-150 / 344.
种属反应性
Mouse, Rat (Predicted: Human)
预测的种属支持售后
验证应用
WB, IHC-P
分子量
Predicted band size: 38 kDa
阳性对照
C6 cell lysate, Mouse ovary tissue lysate, Rat ovary tissue lysate, mouse ovary tissue, rat ovary tissue.
偶联
unconjugated
克隆号
JE31-78
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
靶点
功能
Follistatin, also known as activin-bindings protein, is a protein that in humans is encoded by the FST gene. Follistatin is an autocrine glycoprotein that is expressed in nearly all tissues of higher animals. Its primary function is the binding and bioneutralization of members of the TGF-β superfamily, with a particular focus on activin, a paracrine hormone. An earlier name for the same protein was FSH-suppressing protein (FSP). At the time of its initial isolation from follicular fluid, it was found to inhibit the anterior pituitary's secretion of follicle-stimulating hormone (FSH).
背景文献
1. Bielka W et al. Follistatin and follistatin-like 3 in metabolic disorders. Prostaglandins Other Lipid Mediat. 2023 Dec
2. Li XJ et al. Follistatin promotes LIN28B-mediated supporting cell reprogramming and hair cell regeneration in the murine cochlea. Sci Adv. 2022 Feb
亚细胞定位
Secreted.
别名
Activin binding protein antibody
Activin-binding protein antibody
Follistatin antibody
FS antibody
fst antibody
FST_HUMAN antibody
Human follistatin gene antibody
图片
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Western blot analysis of Follistatin on different lysates with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution.
Lane 1: C6 cell lysate (20 µg/Lane)
Lane 2: Mouse ovary tissue lysate (30 µg/Lane)
Lane 3: Rat ovary tissue lysate (30 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722532) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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