DDX3X Mouse Monoclonal Antibody
Catalog# EM1707-67
DDX3X Mouse Monoclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Monkey
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Rat
概述
产品名称
DDX3X Mouse Monoclonal Antibody
抗体类型
Mouse Monoclonal Antibody
免疫原
Recombinant protein
种属反应性
Human, Mouse, Monkey, Rat
验证应用
WB, IF-Cell, IHC-P, FC
分子量
73/71 kDa
阳性对照
Human DDX3X recombinant protein, DDX3X-hIgGFc transfected HEK293 cell lysate, Hela, NIH3T3, C6, COS7, A431, HEK293, HepG2, human cervical cancer tissue, human ovarian cancer tissue.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
4℃; -20℃ for long term storage.
存储缓冲液
Purified antibody in PBS with 0.05% sodium azide.
亚型
IgG2a
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IF-cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:100-1:200
靶点
功能
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 (DEAD box polypeptide 3) is involved in RNA metabolism. Two DDX3 paralogs are found in humans; DDX3X is encoded by a gene found on the X chromosome while DDX3Y is encoded by a gene on the Y chromosome. DDX3Y is exclusively expressed in testis and is required for normal spermatogenesis. DDX3X is ubiquitously expressed and predominantly localizes to the nuclear speckles, participating in RNA splicing, transcription, translation initiation, mRNA transport and cell cycle regulation. DDX3X also partakes in HIV-1 replication and hepatitis C viral infections.
背景文献
1. Ko C et al. DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis B virus replication at the transcriptional level. J Virol 88:13689-98 (2014).
2. King HA et al. Remodelling of a polypyrimidine tract-binding protein complex during apoptosis activates cellular IRESs. Cell Death Differ N/A:N/A (2013).
亚细胞定位
Nucleus speckle. Cytoplasm.
别名
ATP dependent RNA helicase DDX3X antibody
ATP-dependent RNA helicase DDX3X antibody
CAP Rf antibody
DBX antibody
DDX14 antibody
DDX3X antibody
DDX3X_HUMAN antibody
DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody
DEAD (Asp-Glu-Ala-Asp) box helicase 3
X-linked antibody
展开图片
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Western blot analysis of DDX3X on human DDX3X recombinant protein using anti-DDX3X antibody at 1/1,000 dilution.
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Western blot analysis of DDX3X on HEK293 (1) and DDX3X-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX3X antibody at 1/1,000 dilution.
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Western blot analysis of DDX3X on different cell lysate using anti-DDX3X antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH3T3
Lane 3: C6
Lane 4: COS7
Lane 5: A431
Lane 6: HEK293 -
ICC staining DDX3X (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DDX3X antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DDX3X antibody. Counter stained with hematoxylin.
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Flow cytometric analysis of Hela cells with DDX3X antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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Flow cytometric analysis of HepG2 cells with DDX3X antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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