Asymmetric Di-Methyl Arginine (ADMA) Recombinant Rabbit Monoclonal Antibody [PSH09-55]
Catalog# HA723117
Asymmetric Di-Methyl Arginine (ADMA) Recombinant Rabbit Monoclonal Antibody [PSH09-55]
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ELISA
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WB
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Species independent
概述
产品名称
Asymmetric Di-Methyl Arginine (ADMA) Recombinant Rabbit Monoclonal Antibody [PSH09-55]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Immunogen Asymmetric Dimethylarginine (ADMA) KLH-conjugated
种属反应性
Species independent
验证应用
ELISA, WB
偶联
unconjugated
克隆号
PSH09-55
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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ELISA
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1:2,000
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WB
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1:2,000
靶点
功能
Arginine methylation is a prevalent posttranslational modification which is deposited by a family of protein arginine methyltransferases (PRMTs), and is found in three different forms in mammalian cells: mono-methylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Pan-methylarginine antibodies are critical for identifying proteins that are methylated on arginine residues, and are also used for evaluating signaling pathways that modulate this methyltransferase activity.
背景文献
1. Wu Y et al. Metabolite asymmetric dimethylarginine (ADMA) functions as a destabilization enhancer of SOX9 mediated by DDAH1 in osteoarthritis. Sci Adv. 2023 Feb
2. Ozkarafakili MA et al. Asymmetric Dimethylarginine in COPD Exacerbation. Sisli Etfal Hastan Tip Bul. 2022 Dec
别名
ADMA antibody
Asymmetrical Dimethylarginine antibody
Asymmetric Di-Methyl Arginine antibody
图片
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☑ Cell treatment (CT)
Western blot analysis of Asymmetric Di-Methyl Arginine (ADMA) on different lysates with Rabbit anti-Asymmetric Di-Methyl Arginine (ADMA) antibody (HA723117) at 1/2,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T treated with 10nM Type I PRMT inhibitor MS023 for 48 hours cell lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 1 minute 22 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723117) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Asymmetric Di-Methyl Arginine (ADMA) on different lysates with Rabbit anti-Asymmetric Di-Methyl Arginine (ADMA) antibody (HA723117) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723117) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Indirect ELISA analysis of ADMA.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"