AKR7A2 Mouse Monoclonal Antibody [C10-D5]
Catalog# M1505-12
AKR7A2 Mouse Monoclonal Antibody [C10-D5]
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WB
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IF-Cell
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IHC-P
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Human
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Zebrafish
概述
产品名称
AKR7A2 Mouse Monoclonal Antibody [C10-D5]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Human AKR7A2 aa 97-146 / 359.
种属反应性
Human, Zebrafish
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 40 kDa
阳性对照
HepG2, human spermatozoa
偶联
unconjugated
克隆号
C10-D5
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:200
靶点
功能
Aflatoxin B1 aldehyde reductase member 2 is an enzyme that in humans is encoded by the AKR7A2 gene. It catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate and may have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). AKR7A2 has broad substrate specificity and NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). AKR7A2 can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro) and reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
背景文献
1. "Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of aldo-keto reductase 7 family proteins." Kelly V.P., Sherratt P.J., Crouch D.H., Hayes J.D. Biochem. J. 366:847-861(2002)
2. "Synthesis and catabolism of gamma-hydroxybutyrate in SH-SY5Y human neuroblastoma cells: role of the aldo-keto reductase AKR7A2." Lyon R.C., Johnston S.M., Watson D.G., McGarvie G., Ellis E.M. J. Biol. Chem. 282:25986-25992(2007) "Genetic variation of aflatoxin B1 aldehyde reductase genes (AFAR) in human tumour cells." Praml C., Schulz W., Claas A., Mollenhauer J., Poustka A., Ackermann R., Schwab M., Henrich K.-O.Cancer Lett. 272:160-166(2008)
序列相似性
Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.
组织特异性
Detected in brain, liver, small intestine and testis, and at lower levels in heart, prostate, skeletal muscle and spleen. Detected in kidney proximal and distal tubules, endothelial cells lining the Bowman's capsules and some cysts. Detected at low levels in lung and pancreas (at protein level). Widely expressed.
亚细胞定位
Cytoplasm, Golgi apparatus
UNIPROT
别名
AFAR antibody
AFAR1 antibody
AFB1 aldehyde reductase 1 antibody
AFB1 AR1 antibody
AFB1-AR 1 antibody
AFB1AR1 antibody
Aflatoxin aldehyde reductase antibody
Aflatoxin B1 aldehyde reductase member 2 antibody
Aflatoxin beta1 aldehyde reductase antibody
Aiar antibody
展开图片
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Western blot analysis of AKR7A2 on human spermatozoa lysate using anti-AKR7A2 antibody at 1/1,000 dilution.
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ICC staining AKR7A2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western blot analysis of AKR7A2 on hybrid fish (crucian-carp) liver tissue lysate using anti-AKR7A2 antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"